Agrobacterium-Mediated Transformation of Sunflower (Helianthus Annuus L.) using Direct Shoot Regeneration Protocol


Ya. B. Neskorodov, А. М. Kamionskaya and K. G. Skryabin
Russian Academy of Sciences, Moscow, Russia

Series: Agricultural Research Updates
BISAC: TEC003000

This new book optimizes methods of effective shoots regeneration for a Russian selection of sunflower varieties. A highly effective method of sunflower regeneration in vitro is worked out, where mature embryo (seeds) as explant sources are used. This method enables obtaining four explants with a high regeneration potential from one sunflower seed. The rate of regeneration on the worked out explants ranges from 4583.45 % to 3090.30 % depending on genotype. (Imprint: Novinka)

Table of Contents

Table of Contents


Sterilization method No 1

Sterilization method No 2

Method of Obtaining Explants for Regeneration No 1

Method of Obtaining Explants for Regeneration No 2




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