Table of Contents
Rafael Coveñas, Arturo Mangas, Dominique Bodet, Sebastien Duleu, Pilar Marcos and Michel Geffard
Institute of Neurosciences of Castilla y León (INCYL), Salamanca, Spain, and others
Series: Animal Science, Issues and Research, Neurology – Laboratory and Clinical Research Developments
In general, the metabolic roles played by vitamins are well known, but to date less is known about the anatomical localization of these substances in the mammalian brain. Using a highly specific antiserum directed against vitamin C, the distribution of vitamin C-immunoreactive structures in the monkey (Macaca fascicularis) brain was studied. Antibody avidity and specificity were checked using the ELISA method, and it was estimated that antibody avidity (IC50) was 10-7 M for anti-conjugated ascorbic acid antibodies.
Thus, using an immunoperoxidase technique that increases the immunohistochemical reaction product, no immunoreactive fibers containing vitamin C were found in the monkey brain, but a moderate density of vitamin C-immunoreactive cell bodies was observed in the primary somatosensory cortex (postcentral gyrus). (Imprint: Nova Biomedical)