Various Methods and Novel Techniques: Rapid Molecular Detection of Human, Plant, Genetic, and Microbial Infectious Diseases, Pathogenic Bacteria, and Organisms



: Medical Procedures, Testing and Technology
BISAC: HEA039000; HEA039040

In this book, various methods for detection of infectious diseases in humans and other organisms are described. These include polymerase chain reaction (PCR) technology, loop-mediated isothermal amplification (LAMP), and recombinase polymerase amplification (RPA). Each method presents different strengths and weaknesses and varies by sensitivity, specificity, and rapidity, among other characteristics. These techniques can be used for the detection of pathogens like SARS-CoV, MERS-CoV, SARS-CoV-2, influenza, lymphocystis disease virus, swine acute diarrhea syndrome coronavirus, swine vesicular disease virus, classical swine fever virus, infectious bursal disease virus, Marek’s disease virus, human papillomaviruses, infectious bronchitis virus, Newcastle disease virus, sacbrood virus, beak and feather disease virus, foot-and-mouth disease virus, bovine herpesvirus-1, and more. Other applications of these methods include molecular diagnosis of cancer, identification of genetically modified organisms, and detection of food adulteration.

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Table of Contents


About the Authors

Chapter 1. Introduction

Chapter 2. Molecular Detection

Chapter 3. Molecular Detection and Epidemic Diseases

Chapter 4. Molecular Detection of COVID-19

Chapter 5. Rapid Detection

Chapter 6. Rapid Detection of COVID-19

Chapter 7. PCR

Chapter 8. Loop-Mediated Isothermal Amplification

Chapter 9. Rapid Disease Detection

Chapter 10. PCR and Epidemic Diseases

Chapter 11. PCR and Covid-19

Chapter 12. Advantages and Disadvantages of Molecular Detection

Chapter 13. Recombinase Polymerase Amplification (RPA)

Chapter 14. Recombinase Aided amplification (RAA)


Author’s ORCID iD

Wenli Sun
Mohamad Hesam Shahrajabian

Additional information


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