The Influence of Different Detergents on the Preservation of Discrete Membrane Microdomains: A Comparative Ultrastructural Study on Whole-Mounted Colorectal Cancer Cells

Kristina A. Jahn, Yingying Su, Tessa Smissaert van de Haere, Iris Benjamins and Filip Braet (Editors)
The University of Sydney, Australia

Series: Cancer Etiology, Diagnosis and Treatments
BISAC: MED062000

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Volume 10

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Special issue: Resilience in breaking the cycle of children’s environmental health disparities
Edited by I Leslie Rubin, Robert J Geller, Abby Mutic, Benjamin A Gitterman, Nathan Mutic, Wayne Garfinkel, Claire D Coles, Kurt Martinuzzi, and Joav Merrick

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The cellular plasma membrane is partially resistant to solubilisation with mild and non-denaturing detergents allowing detergent-resistant membranes (DRMs) to be isolated and biochemically analysed. The composition of DRMs however varies depending on the detergent applied.

A previously developed novel method to visualise DRMs on whole-mount cancer cells in which the soluble fraction of the plasma membrane is removed and the cytoskeleton is fixed and prepared for scanning electron microscopy is reviewed. This method is less destructive than many others commonly used to study DRMs and it enables the direct visualisation of intact, cytoskeleton-associated DRMs. This new book reviews new and valuable ultrastructural information at the nanometer scale that did not exist to date. (Imprint: Nova Biomedical)

ABSTRACT

INTRODUCTION

MATERIALS AND METHODS

RESULTS AND DISCUSSION

CONCLUSION

REFERENCES

INDEX

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