The Handbook of Food Microbiological Analytical Methods

Ismail Mohamed Al Bulushi, Ph.D.
College of Agricultural and Marine Sciences, Sultan Qaboos University, Oman

Series: Microbiology Research Advances
BISAC: SCI045000



Volume 10

Issue 1

Volume 2

Volume 3

Special issue: Resilience in breaking the cycle of children’s environmental health disparities
Edited by I Leslie Rubin, Robert J Geller, Abby Mutic, Benjamin A Gitterman, Nathan Mutic, Wayne Garfinkel, Claire D Coles, Kurt Martinuzzi, and Joav Merrick


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The Handbook of Food Microbiological Analytical Methods includes 31 topics and experiments distributed throughout five chapters, namely basic microbial skills, the enumeration of different microorganisms in foods, identification techniques and determination of microbial activities. Besides, the handbook includes useful sources in food microbial analyses such as the Food Microbiology E-sources and the primers for identification of common microbial pathogens. This handbook aims to provide and develop concerning food microbial skills in the users with simple steps to follow along with the theoretical explanation for better understanding.

The uniqueness of this handbook includes topics that are rarely addressed in current food microbiology manuals and handbooks such as the enumeration of special bacterial groups such as marine and Gram-positive bacteria, introducing: A selective medium for enumeration of Gram-positive bacteria from marine sources for the first time; a technique for bacterial colonies randomization; a technique for recovery of injured/stressed bacteria; techniques to study bacterial potential such as spoiling foods; a technique that forms biogenic amines; and the production of antimicrobial activity and providing the specific specie primer pairs for common pathogens.

The principles and procedures of some routinely used identification techniques – namely vitek, sequencing of 16S rRNA gene and specific genus and specie primers for bacterial identification – are provided with simple procedures. Enumeration and identification techniques of common pathogens and spoilers, namely Staphylococcus aureus, Enterobacteriaceae, Escherichia coli, Salmonella spp., Listeria monocytogenes, Vibrio parahaemolyticu, Bacillus cereus and Shewanella putrefaciens enumeration and identification techniques are also covered.

These techniques are based on the International Standards such as ISO and U.S. Food and Drug Administration Standards and the media’s manufacture instructions.

This handbook is needed to conduct microbial analyses to determine food microbial quality and safety, food hygienic status and the microbial potential to spoil food, lose food safety and produce antimicrobial activity. This handbook was prepared to be used by students and young researchers. Therefore, it can be used in the universities as a practical manual in biology, microbiology, food microbiology, food safety, and food hygiene courses, as well in food laboratories which determine food microbial safety and quality. It is expected that this handbook will be a good and practical guide for students and researchers as well. (Imprint: Nova)



About the Author

Chapter 1. Basic Microbial Skills (pp. 1-40)

Chapter 2. Enumeration of Specific Microorganisms in Foods (pp. 41-70)

Chapter 3. Enumeration and Identification of Pathogens in Foods (pp. 71-100)

Chapter 4. Microbial Identification Techniques (pp. 101-114)

Chapter 5. Determination of Microbial Activities (pp. 115-130)

References (pp. 131-136)

Appendix (pp. 137-140)

Index (pp. 141)

Total Pages: 147

“The Handbook of Food Microbiological Analytical Methods is a well written text that will prove of use to individuals in academia, research and industry. It describes clear and unambiguous methodology for conducting a range of techniques in the food microbiology laboratory. It has an easy to follow format that will lend itself to use by students or by others learning about food microbiology. It provides enough theory for the basis of the methods to be understood and also a range of links to resources where further information is available. I feel that the text is an excellent addition to the available literature and should be on the bookshelf of every food microbiologist.” - Professor Gary Dykes, School of Public Health, Curtain University, Western Australia

Al Bulushi 2016. Laboratory Manual. Muscat: Sultan Qaboos University Press.
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Audience: - Under graduate and graduate students from Food Science and Biotechnology majors in colleges and universities
- Food Science Researchers.
- Other science researchers working in biology, marine, animal, soil and crops fields
- Food Microbiology/ Food Science/ Biology Technicians
- Food safety and hygiene authorities

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