Stem Cells: Mediated Regeneration


Prasad S. Koka (Editor)
Chief Scientific Director and Professor of DiponEd Institute of Regenerative Medicine Merisis Therapeutics – DiponEd BioIntelligence, Bangalore, India

Series: Stem Cells – Laboratory and Clinical Research
BISAC: SCI017000

Stem cells of varying potencies originating from the organ generating totipotent embryonic stem cells (ESCs) to terminally differented cells such as platelets formation, via pluripotent hematopoietic stem cells (HSCs), and their downstream multipotent mesenchymal stem cells (MSCs), have in general been well characterized. Thus majority of these different stem cell types and their differentiation ‘progeny’ can be tracked for subjecting to further characterizations for clinical relevance and consequently needed biological modifications at the cellular and molecular levels, for patients’ clinical treatments. The very first section of this book’s contents deal with the above stated description.

Such characterizations and research laboratory manipulations of the stem cells have led to their clinical grade applications and treatments with positive benefits, as described in the second section of this book. The clinical benefits as published by Nova Science Publishers herein as part of the contents are of tremendous significance and provide relief to the handicapped individuals, particularly for those who suffered life’s biological hazards. This naturally seeks R&D-mediated bench to bedside benefits and solutions for the genetically defective individuals.

Bone marrow-derived stem cells with HSCs as their primary components are increasingly being cast the human eyes with pregnant females resorting cord blood banking as they peek to dwell into the future well being of their offspring(s) which could even benefit their own selves, should there be expectedly sufficient HLA matching. The third section of this book dels with such topics. The induced pluripotent stem cells (iPSCs) which were generated to overcome the ethical considerations of using ESCs were fraught with ‘elaborate’ molecular and cellular biological engineering methods and the advent of derivation and usefulness of MSCs have since sidelined or relegated the iPSCs to a distant role. Nonetheless the hitherto served purpose of iPSCs is not discounted and yet unknown stumbling blocks of what seems to be a smooth going ‘runway’ of MSCs may spring up in the clinical paths necessitated by the treatment along the future.

In the fourth section of the book, it is to be kept in mind that uncontrolled proliferation of cells should be avoided at all costs. Whereas self-renewal of stem cells is generally expected to lead to a turnover, it need not be the case with regeneration processes. This will depend upon the genetic make up of the donor-derived and invasively injected stem cells plus accompanying biological reagent components, as well as also the recipients’ physical conditions.

The fifth and last section of the book deals with conventional and alternative medicine topics applicable to carcinogenesis or such tendencies. Telomerase activity is a ‘double edged sword’. Therefore its homeostasis or equilibrium is to be maintained. Discriminatory apoptosis that is of beneficial nature affecting malignant cells but not the normal cells is necessary. Thus equation between apoptotic and telomerase activities even those of stem cells should be as hand in glove. (Imprint: Nova Biomedical)



Table of Contents

Preface: Regeneration Restricted to Non-Carcinogenicity

Section 1: Manipulation and Characterization of Stem Cells

Chapter 1. Degeneration versus Regeneration
Prasad S Koka

Chapter 2. Differentiation Potential of Mesenchymal Stem Cells from Equine Bone Marrow Cultured on Hyaluronic Acid-Chitosan Polyelectrolyte Multilayer Biofilm
Amanda J. Listoni, Isadora Arruda, Leandro Maia, Danielle J. Barberini, Ian Martins, Fernando C. Vasconcellos, and Fernanda C. Landim-Alvarenga

Chapter 3. Stem Cells Cultured on Beta Tricalcium Phosphate (¦Â-TCP) in Combination with Recombinant Human Platelet-Derived Growth Factor ¨C BB (rh-PDGF-BB) for the Treatment of Human Infrabony Defects
Roshani Dhote, Priti Charde, Manohar Bhongade, and Jyotsana Rao

Chapter 4. Fat Layer from Medullary Canal Reamer Aspirate for Potential Use as a Supplemental Osteoinductive Bone Graft Material
Sarina S. Kay Sinclair, C. Olsen Horton, Kyle J. Jeray, Stephanie L. Tanner, and Karen J. L. Burg

Chapter 5. Deprivation of bFGF Promotes Spontaneous Differentiation of Human Embryonic Stem Cells into Retinal Pigment Epithelial Cells
Lee R. Ferguson, Sankarathi Balaiya, Bharani K. Mynampati, Kumar Sambhav, and Kakarla V. Chalam

Chapter 6. Stability of Reference Genes During Tri-Lineage Differentiation of Human Adipose-Derived Stromal Cells
Luciana Fraga da Costa Diesel, Bruno Paiva dos Santos, Bruno Corr¨ºa Bellagamba, Angelo Syrillo Pretto Neto, Pedro Bins Ely, Lindolfo da Silva Meirelles, Nance Beyer Nardi, and Melissa Camassola

Chapter 7. Anaerobic Glycolysis and HIF1alpha Expression in Haematopoietic Stem Cells Explains Its Quiescence Nature
Lokanathan Srikanth, Manne Mudhu Sunitha, Katari Venkatesh, Pasupuleti Santhosh Kumar, Chodimella Chandrasekhar, Bhuma Vengamma, and Potukuchi Venkata Gurunadha Krishna Sarma

Section 2: Regeneration Treatments with Stem Cells

Chapter 8. Mesenchymal Stem Cells and Lung Transplantation: A Couple for a Perfect Relationship
Mohamed S. A. Mohamed

Chapter 9. Healing of Experimentally Created Non-Union of Femur in Rats Using Bone Precursor Cells from Mesenchymal Stem Cells (MSCs)
Mir Sadat-Ali, Md Quamar Azam, Dakheel A. Al-Dakheel, and Sadananda Acharya

Chapter 10. Human Umbilical Cord Mesenchymal Stem Cells in the Treatment of Duchenne Muscular Dystrophy: Safety and Feasibility Study in India
B. S. Rajput, Swarup K. Chakrabarti, Vaishali S. Dongare, Christina M. Ramirez, and Kaushik D. Deb

Chapter 11. Therapeutic Application of Bone Marrow-Derived Stem Cells in a Patient with Methanol-Induced Blindness
Himanshu Bansal, Anupama Bansal, M. Neelam Kachhap, Abhay Chowdhary, and Prasad S. Koka

Section 3: Hematopoietic Stem Cells

Chapter 12. The Impact Age, Sex, and Religious Beliefs have on the Attitude towards Cord Blood Banking
Inger Birgitta Sundell and Teddi J. Setzer

Chapter 13. Hematopoietic Stem Cells, Their Niche, and the Concept of Co-Culture Systems: A Critical Review
Anuradha Vaidya and Vaijayanti Kale

Chapter 14. Mechanism of Induction: Induced Pluripotent Stem Cells (iPSCs)
Vimal Kishor Singh, Neeraj Kumar, Manisha Kalsan, Abhishek Saini, and Ramesh Chandra

Section 4: Stem Cells and Carcinogenesis

Chapter 15. Regeneration and Carcinogenesis
A. V. Pechersky, V. I. Pechersky, A. B. Smolyaninov, V. N. Velyaninov, S. F. Adylov, A. Yu. Shmelev, O. V. Pecherskaya, and V. F. Semiglazov

Chapter 16. Cancer Stem Cells and Chemoresistance in Glioblastoma Multiform: A Review Article
Mojdeh Safari and Alireza Khoshnevisan

Chapter 17. Effect of Mobile Phone-Induced Electromagnetic Field on Brain Hemodynamics and Human Stem Cell Functioning: Possible Mechanistic Link to Cancer Risk and Early Diagnostic Value of Electronphotonic Imaging
Hemant Bhargav, T. M. Srinivasan, S. Varambally, B. N. Gangadhar, and Prasad Koka

Section 5: Mechanisms of Prevention of Stem Cells-Mediated Carcinogenic Trends

Chapter 18. Imiquimod Treatment Effectively Reduces the Percentage of Viable Cells in a Cervical carcinoma Cell Line But Does Not Affect the Expression of HLA-G or OCT-4
Konstantinos Stefanidis, Jessica Patta, Vasilios Pergialiotis, Diamanto Stefanidi, and Dimitrios Loutradis

Chapter 19. Antiaging Effects of an Intensive Mind and Body Therapeutic Program through Enhancement of Telomerase Activity and Adult Stem Cell Counts
Krishna S. Rao, Swarup K. Chakrabarti, Vaishali S. Dongare, K. Chetana, Christina M. Ramirez, Prasad S. Koka, and Kaushik D. Deb


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