SPR-Facilitated Interrogation of Enzymatic Reactions Involving Nucleic Acids

Wendi M. David
Texas State University-San Marcos, San Marcos, TX, USA

Series: Protein Biochemistry, Synthesis, Structure and Cellular Functions
BISAC: MED060000

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Special issue: Resilience in breaking the cycle of children’s environmental health disparities
Edited by I Leslie Rubin, Robert J Geller, Abby Mutic, Benjamin A Gitterman, Nathan Mutic, Wayne Garfinkel, Claire D Coles, Kurt Martinuzzi, and Joav Merrick

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Details

Analytical techniques designed to monitor intermediate or real-time interactions include fluorescence resonance energy transfer (FRET) spectroscopy and surface plasmon resonance (SPR). SPR is label-free, an advantage for interrogating many biomolecular interaction events. Although SPR has been used extensively to determine small molecule/DNA and protein/DNA binding interactions, SPR analysis of enzymatic reactions involving nucleic acids has lagged behind. However, several recent studies of enzymatic reactions that extend nucleic acids or unwind different topological forms of DNA demonstrate significant advantages of SPR for discerning aspects of these interactions that are difficult to “see” using other techniques. This book focuses on the emerging application of SPR technology for elucidating enzymatic reactions involving nucleic acids. (Imprint: Nova)

ABSTRACT

INTRODUCTION

DIRECT MEASUREMENT OF ENZYMATIC ACTIVITY INVOLVING OLIGONUCLEOTIDES

ENZYME-BASED STRATEGIES FOR INCREASING NUCLEIC ACID DETECTION SENSITIVITY

CONCLUSION

REFERENCES

INDEX

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