Recent Advances in Proteomics Research


Ming D. Li, PhD (Editor)
Department of Psychiatry and NB Sciences, University of Virginia, Charlottesville, VA, USA

Series: Protein Biochemistry, Synthesis, Structure and Cellular Functions
BISAC: SCI007000

The primary objective of this book is to provide updated knowledge of recent advances in proteomics research in various fields. The topics covered include, but are not limited to, proteomic analysis for microbiomes, specific cell types from heterogeneous tissues, body fluids, mitochondria, and environmental toxicology. Almost all proteomics techniques currently used in research have been reviewed in at least one chapter, including traditional one- or two-dimensional gel-based, gel-free shotgun, label-based or label-free quantitative, and array-based methods. In addition, although they are not the major focus of this book, proteomics-related bioinformatics tools such as database searching, data mining, data analysis, and gene pathway analysis are briefly covered.

Additionally, this book contains chapters on some technical issues related to applying proteomics technologies to specific issues, such as sample preparation for removal of contaminants and depletion of highly abundant proteins. Collectively, these chapters provide a broad view of various technologies commonly used in proteomics research and their successful applications to address different questions across various species. It is our hope that such a book can provide readers with a comprehensive understanding of proteomics technologies and their application to address various scientific questions. (Imprint: Nova Biomedical )




Table of Contents

Preface pp,vii-viii

Chapter 1. Protein Level Analysis from Specific Cell Types in Heterogeneous Tissue
(Andrew O. Koob, University of Wisconsin – River Falls, Wisconsin, USA)pp,1-34

Chapter 2. Neuroproteomics and its Application in Drug Addiction Study
(Ju Wang, Ming D. Li, School of Biomedical Engineering, Tianjin Medical University, China, and others)pp,35-72

Chapter 3. Current Proteomics Methods For Analyzing the Human Microbiome
(Luis Pinto, Patrícia Poeta, Concha Gil, Gilberto Igrejas, Department of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal, and others)pp,73-104

Chapter 4. Analysis of Pleural Fluid: The Path Travelled with Proteomic Guidance
(Suchit Kumar Chatterji, Pao-Chi Liao, Department of Environmental and Occupational Health, College of Medicine, National Cheng Kung University, Tainan, Taiwan)pp,105-118

Chapter 5. Application of Quantitative Proteomics to Disease Diagnosis and Prognosis Using Stable Isotope Dimethyl Labeling Coupled with Shotgun Proteomics Strategy and Multiple Reaction Monitoring
(Thomas Shyh-Horng Chiou, Min-Yi Lee, Chao-Jen Kuo, Shih-Shin Liang, Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Taiwan, and others)pp,119-138

Chapter 6. Mitochondria Protein Profiling in Striated Muscle: Insights into Pathophysiological Conditions
(Ana Isabel Padrão, Rui Vitorino, Rita Nogueira-Ferreira, Vanessa Almeida, Tiago Henriques-Coelho, José A. Duarte, Francisco Amado, Rita Ferreira, QOPNA, Mass Spectrometry Group, University of Aveiro, Aveiro, Portugal, and others)pp,139-174

Chapter 7. Structural Mass Spectrometry of Halophilic Proteasomal Nanomachines Using Chemical Cross-Linking
(Julie Maupin-Furlow, Ivanka Karadžić, David Goodlett, Department of Microbiology and Cell Science, University of Florida, Gainesville, Florida, USA, and others)pp,175-200

Chapter 8. Introduction to Protein Arrays and their Application to Nicotine Research in Macrophages
(Wen-Yan Cui, Ming D. Li, State Key Laboratory for Diagnosis and Treatment of Infections Diseases, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China, and others)pp,201-218

Chapter 9. Quantitative Proteomic Approaches for Environmental Toxicology
(John M. Prins, Yinsheng Wang, D.I. Lurie, Department of Chemistry, University of California, Riverside, California, USA, and others)pp,219-238

Chapter 10. Toxin Proteomics for the Identification and Differentiation of Botulinum Neurotoxins
(Suzanne R. Kalb, John R. Barr, Centers for Disease Control and Prevention, National Center for Environmental Health, Division of Laboratory Sciences, Atlanta, Georgia, USA)pp,239-254

Chapter 11. Challenges of Mining Low to Medium Abundant Proteins for Proteomics
(Balamurugan Natarajan, Rahim Abdullah, Nazrul Islam and Savithiry S. Natarajan, University of Maryland-College Park, Maryland, USA and others)pp,255-270

Index pp,271-286

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