Reactive Oxygen Species, Lipid Peroxidation and Protein Oxidation


Angel Catalá, PhD (Editor)
Facultad de Ciencias Exactas, Universidad Nacional de La Plata, CONICET, CIC., La Plata, Argentina

Series: Chemistry Research and Applications
BISAC: SCI017000

Many studies have shown that free radical damage and lipid peroxidation increase as a function of the degree of unsaturation of the fatty acids present in the phospholipids of biological membranes. Membrane phospholipids are particularly susceptible to oxidation not only because of their highly polyunsaturated fatty acid content but also because of their association in the cell membrane with non-enzymatic and enzymatic systems capable of generating prooxidative-free radical species.

There are two broad outcomes to lipid peroxidation: structural damage to membranes and generation of secondary products. Membrane damage derives from the generation of fragmented fatty acyl chains, lipid–lipid cross-links, lipid–protein cross-links and endocyclization to produce isoprostanes and neuroprostanes. These processes combine to produce changes in the biophysical properties of membranes that can have profound effects on the activity of membrane-bound proteins. The consequence of peroxidation of unsaturated fatty acids is severe: damage of membranes function, enzymatic inactivation, toxic effects on the cellular division, etc.

This new book presents and discusses current research on oxidative stress, and lipid oxidation – non-inhibited and inhibited; assessment of oxidative balance in the lipo- and hydro-philic cellular environment in biological systems; mass spectrometry detection of protein modification by cross-reaction with lipid peroxidation products; diagonal gel electrophoretic analysis of protein disulfides: principles and applications. Other chapters describe: heavy metals exposure and cells oxidative damage; biodegradation of metallic biomaterials: its relation with the generation of reactive oxygen species; Oxidative modifications of proteins in the aging heart; role of reactive oxygen species as signalling molecules involved in the regulation of physiological processes of the nervous system; oxidative stress in diabetes and hypertension treated with alternative therapy of medicinal plants; impairment of redox homeostasis of tissue damage in inborn errors of metabolism with intoxication: insights from human and animal studies and finally lipid peroxidation of phospholipids in the vertebrate retina or in liposomes made of retinal lipids: similarities and differences are described. (Imprint: Nova)



Table of Contents


Chapter 1 – Oxidative Stress and Lipid Oxidation –Non-Inhibited and Inhibited (pp. 1-42)
Vessela D. Kancheva (Lipid Chemistry Department, Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Sofia 1113, Bulgaria)

Chapter 2 – Assessment of Oxidative Balance in the Lipo- and Hydro-Philic Cellular Environment in Biological Systems (pp. 43-60)
G. Malanga, J. M. Ostera and S. Puntarulo (Physical Chemistry-Institute of Biochemistry and Molecular Medicine (IBIMOL), School of Pharmacy and Biochemistry, University of Buenos Aires-CONICET, Junín 956 (C1113AAD) Buenos Aires, Argentina)

Chapter 3 – Mass Spectrometry Detection of Protein Modification by Cross-Reaction with Lipid Peroxidation Products (pp. 61-86)
M. Rosário Domingues, Maria Fedorova and Pedro Domingues (Mass Spectrometry Centre, QOPNA, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal and others)

Chapter 4 – Diagonal Gel Electrophoretic Analysis of Protein Disulfides: Principles and Applications (pp. 87-94)
Xiaoting Luo, Rongrong Li and Liang-Jun Yan (Department of Biochemistry and Molecular Biology, Gannan Medical University, Ganzhou, Jiangxi Province, 341000, China and others)

Chapter 5 – Participation of Reactive Oxygen Species in the Toxicity of Cobalt, Nickel, Cadmium and Mercury (pp. 95-126)
Sandra Viviana Verstraeten (Department of Biological Chemistry, IQUIFIB (UBA-CONICET), School of Pharmacy and Biochemistry, University of Buenos Aires, Argentina)

Chapter 6 – Biodegradation of Metallic Biomaterials: Its Relation with the Generation of Reactive Oxygen Species (pp. 127-140)
Natalia S. Fagali, Claudia A. Grillo, Susana Puntarulo and Mónica A. Fernández Lorenzo (Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA), CCT La Plata – CONICET, Dpto. de Química, Fac. de Cs. Exactas, UNLP, CC 16, Suc.4, 1900, La Plata, Buenos Aires, and Instituto de Bioquímica y Medicina Molecular (IBIMOL), FFyB, UBA, Junin 956, 2º piso, CABA, Buenos Aires, Argentina (3)Facultad de Ingeniería, UNLP, Calle 47 y 1, 1900, La Plata, Buenos Aires, Argentina)

Chapter 7 – Oxidative Modifications of Proteins in the Aging Heart (pp. 141-168)
Peter Kaplan, Zuzana Tatarkova, Veronika Ilovska, Jan Lehotsky, Peter Racay and Dusan Dobrota (Department of Medical Biochemistry, Jessenius Faculty of Medicine, Comenius University, Martin, Slovak Republic)

Chapter 8 – Role of Reactive Oxygen Species As Signaling Molecules in the Regulation of Physiological Processes of the Nervous System (pp. 169-204)
Mauricio Olguín-Albuerne, Marco Antonio Zaragoza-Campillo and Julio Morán (Departamento de Neuropatología Molecular, División de Neurociencias, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, México, D.F. CP 04510, Mexico)

Chapter 9 – Oxidative Stress in Diabetes and Hypertension Treated with Alternative Therapy of Medicinal Plants (pp. 205-218)
Alfredo Saavedra-Molina, Rafael Salgado-Garciglia, Ruth Noriega-Cisneros, Edgar R. Esquivel-Gutiérrez, Salvador Manzo-Avalos, Christian Cortés-Rojo and Rocío Montoya-Pérez (Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo. Morelia, Mich. 58030. México)

Chapter 10 – Redox Homeostasis Impairment as an Important Pathomechanism of Tissue Damage in Inborn Errors of Metabolism with Intoxication: Insights from Human and Animal Studies (pp. 219-242)
Guilhian Leipnitz, Bianca Seminotti, César Augusto João Ribeiro and Moacir Wajner (Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, and Serviço de Genética Médica, Hospital de Clínicas de Porto Alegre, Porto Alegre, RS, Brazil)

Chapter 11 – Lipid Peroxidation of Phospholipids in Retinal Membranes and in Liposomes Made of Retinal Lipids: Similarities and Differences (pp. 243-254)
Angel Catalá (Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas, (INIFTA-CCT La Plata-CONICET), Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Argentina)


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