Protein Engineering: Design, Selection and Applications


Mallorie N. Sheehan (Editor)

Series: Protein Biochemistry, Synthesis, Structure and Cellular Functions
BISAC: SCI013000

Protein engineering is the process of developing useful or valuable proteins. It is a young discipline, with much research currently taking place into the understanding of protein folding and protein recognition for protein design principles. There are two general strategies for protein engineering. The first is known as rational design, in which the scientist uses detailed knowledge of the structure and function of the protein to make desired changes.

The second strategy is known as directed evolution and this is where random mutagenesis is applied to a protein, and a selection regime is used to pick out variants that have the desired qualities. This new book presents and reviews important data on protein engineering, such as application of engineered proteins and cell adhesive surfaces as scaffolds or other biomedical devices which has the potential to promote tissue repair and regeneration for a wide variety of tissues including bone and skin. (Imprint: Nova)



Table of Contents

Preface pp. i-xii

Synthesizing Mammalian Cell Adhesive Surfaces using Self-Assembling Fibronectin Domains
(Patricia Pereira, Christopher F. van der Walle, iMed.UL – Research Institute for Medicines and Pharmaceutical Sciences, Faculdade de Farmácia, Universidade de Lisboa, Lisboa, Portugal, and Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, Scotland)pp. 1-28

Engineering Thermobifida fusca Cellulases: Catalytic Mechanisms and Improved Activity
(Thu V. Vuong, David B. Wilson, Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York, USA)pp. 29-52

An Empirical Study for Finding a Robust Ensemble of Classifiers for Protein Classification
(Loris Nanni, Alessandra Lumini, DEIS, IEIIT—CNR, Università di Bologna, Bologna, Italy)pp. 53-72

Revolutionary Protein Engineering Using Molecular Display
(Mitsuyoshi Ueda, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo, Kyoto, Japan)pp. 73-80

Chemoselective Modification using Aldehyde/ketone Tags
(Koichiro Kodama, Seketsu Fukuzawa, Department of Chemistry, School of Science, The University of Tokyo, Hongo, Tokyo, Japan)pp. 81-88

Computer Modeling of the Promising HIV-1 Entry Inhibitors for Structure-Based Anti-AIDs Drug Design: Cyclophilin b and gp120 v3 Loop ^
(Alexander M. Andrianov, and Ivan V. Anishchenko, Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus, Minsk, Republic of Belarus)pp. 89-126

Molecular Mechanisms of Protein Folding by Mammalian ER Chaperones and Stress Response
(J. Geraldine Sandana Mala, Chellan Rose and Satoru Takeuchi; Department of Biotechnology, Central Leather Research Institute, Chennai, India and Factory of Takeuchi Nenshi, TAKENEN, Takamatsu, Kahoku, Ishikawa, Japan)pp. 127-162

The Use of Protein Engineering Methods for Studies of Calcium Binding Proteins
(Eugene A. Permyakov, Vladimir N. Uversky, Institute for Biological Instrumentation of the Russian Academy of Sciences, Moscow, Russia, and others)pp. 163-204

Index pp. 205-223

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