Table of Contents
1. In vitro proliferation of Treg is dependent on exogenous IL-2 or the responding cells.
2. Feedback loop of immune regulation by Tregs
3. Tregs proliferate well in vitro as well as in vivo.
4. Distinct mode of suppression according to the strength of signal
5. Antigen presenting cells (APCs) and APC-free system
6. Pitfalls of in vitro suppressive assay
7. An alternative quantitative suppressive assay