Table of Contents

ABSTRACT

Industrial use of microbial enzymes has increased greatly in 21st century and continuously increases due to their significant potential. These microbial enzymes have gained global recognition for their widespread use in various sectors of industries. Naringinase, a multienzyme complex, possesses α-L-rhamnosidase and β-glucosidase active centers. Naringinase has debittering properties and have applied commercially for its ability to breakdown the compound naringin in citrus juices which confers a bitter taste. Firstly, rhamnosidase breaks naringin into prunin and rhamnose, followed by breaking prunin into glucose and naringenin by glucosidase. Naringinase had gained a great attention in recent years due to its numerous applications and safety. Hydrolytic activities of naringinase include production of rhamnose, prunin, and debittering of citrus fruit juices. Fungal naringinases have been extensively studied during the past decade and used industrially in large amounts. These enzymes improved the trend of usage as organic based feed additives which create a new ground in organic agriculture. This decreases the usage of synthetic chemicals in animal and could have an appreciable impact on the national gross domestic product if adopted in agro-allied industry. Further research should be directed towards biomolecular engineering to get strains with high specificity and action directed for high safety degree.

Keywords: naringinase, α-L-rhamnosidase, debittering, feed additives, microbial enzymes