Micropropagation: Methods and Effects

Valdir M. Stefenon, Ph.D. (Editor)
Nucleus of Molecular Ecology and Plant Micropropagation, Universidade Federal do Pampa, São Gabriel, RS, Brazil

Series: Plant Science Research and Practices
BISAC: SCI011000

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Plant micropropagation is one of the most classical and widespread biotechnological tools used around the world. Undoubtedly, this technique brought quite important advances to our knowledge about morphological, physiological and developmental patterns of plants, to the progress of genetic breeding and to the establishment of the genetic engineering, among others.

This technique is based on the “in vitro farming” of plant fragments in an axenic environment, where all nutrients needed are kindly provided to the cultivated plant, which is maintained under controlled temperature and light-darkness periods. Although it can be considered an established technique, several advances have been integrated to the routines of micropropagation laboratories each year, incorporated to the productive sector and to the academy.

In Micropropagation: Methods and Effects, the authors aimed to shortly present some of these advances, as well as practical results of using this biotechnology towards the conservation of plant genetic resources. In seven chapters, methods employed for the micropropagation of different plant species, from agricultural cultures to tree species of the Brazilian Atlantic Forest and advances in techniques for explants disinfection and for analysis of stomata complexes, as well as the progresses related to the micropropagation of apple and the use of bioreactors are presented.

The seven chapters of this book will contribute towards incorporating new ideas to the daily effort of each reader, reaching the basic requests of students, professors, researchers and even non-specialized readers.
(Imprint: Nova)

Preface

Chapter 1. Applications of Micropropagation in Plant Biotechnology
(André Luís Lopes da Silva, Paula Kielse, Micheli Angelica Horbach, Gilvano Ebling Brondani, Juline Marta Walter, Leandro Freire dos Santos, Peyman Habibi, Jairton Fraga Araújo, Department of Technology and Social Sciences, State University of Bahia, Juazeiro, Brazil, and others)

Chapter 2. Micropropagation Strategy Focused on the Conservation of Genetic Resources: Aspects of Some Species from the Brazilian Atlantic Rainforest
(Lia Rejane Silveira Reiniger, Aline Ritter Curti, Charlene Moro Stefanel, Diego Pascoal Golle3, Karol Buuron da Silva, Silvia Machado Rabaiolli and Tatiane Laís Pires Andreolla, Universidade Federal de Santa Maria, Santa Maria, Brazil, and others)

Chapter 3. When Micro Becomes Nano: Using Nanoparticles in Micropropagation
(Paulo Roberto Diniz da Silva, Fernanda Illamas Gallon, Marina Junges, Denise Czarnescki da Silva and Suelen Martinez Guterres, Universidade Federal do Pampa, Campus São Gabriel, Brazil, and others)

Chapter 4. Temporary Immersion Bioreactors: Cultivation of Sweet Potato from Meristems
(Diego Pascoal Golle, Jana Koefender, Juliane Nicolodi Camera, Cristiane Kaiper and Roberta Cattaneo Horn, Universidadede de Cruz Alta, Cruz Alta, Brazil)

Chapter 5. Microscopic Evaluation of Stomatal Complex in in Vitro Propagated Plants
(Paulo Roberto Diniz da Silva, Ferdinando Bisogno de Castro, Tatiana Silveira de Moura, Denise Czarnescki da Silva, João Rafael and Larissa Farias Leite, Universidade Federal do Pampa, Campus São Gabriel, Brazil)

Chapter 6. Tissue Cultures in Apples and Their Implications in Genetic Breeding: A Review
(Adriana Cibele de Mesquita Dantas and Valdir Marcos Stefenon, Universidade Estadual do Rio Grande do Sul, Caxias do Sul, Brazil, and others)

Chapter 7. Molecular Markers for Analysis of Genetic Stability in Tissue Culture of Forest Species
(Diego Pascoal Golle, Lia Rejane Silveira Reiniger, Jana Koefender and Roberta Cattaneo Horn, Universidadede de Cruz Alta, Cruz Alta, Brazil, and others)

Index

Keywords: Plant Micropropagation, Biotechnology, Plant Sciences

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