Introduction to Flow Cytometry

Jakub Werner (Editor)

Series: Cell Biology Research Progress
BISAC: SCI017000

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Volume 10

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Special issue: Resilience in breaking the cycle of children’s environmental health disparities
Edited by I Leslie Rubin, Robert J Geller, Abby Mutic, Benjamin A Gitterman, Nathan Mutic, Wayne Garfinkel, Claire D Coles, Kurt Martinuzzi, and Joav Merrick

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Introduction to Flow Cytometry first discusses the general principles of flow cytometry. This technique continues to be developed and is used in many medical applications.

The authors discuss the condition of cell suspension which is entrained in the center of stream of liquid.

Additionally, the most common usage and selected applications of flow cytometry in clinical practice is presented.

In recent years, thanks to the use of new generation dyes, the cytometry has a much higher sensitivity and specificity and allows for the simultaneous registration of more parameters, which leads to a huge amount of information from a single experiment.

Selected techniques of flow cytometry dedicated to measuring DNA content are reviewed. Flow cytometry is used to estimate DNA content in individual cells in large cell populations.

Flow cytometry measures changes in the quality and quantity of specific cells. As such, flow cytometer-associated software for analysis of large data sets is examined.

Parameters and probes used in this technique are also discussed.

Next, the authors discuss the application of flow cytometry in the study of cells in normal blood and bone marrow.
The application of flow cytometry to acute leukaemia diagnosis is explored. This diagnostic method is prerequisite for individual treatment strategies and for the evaluation of treatment response.

Following this, the application of flow cytometry to disorders of plasma celldiagnosis is discussed.

This compilation similarly explores the evolution of the crossmatch assay and the important factors to take into consideration while performing, as well as interpreting results of this fundamental assay for the fate of the transplanted organ.

The penultimate chapter mainly focuses on comparing cytometric bead array to ELISA, which is considered the “gold standard” for soluble molecules determination.

In closing, the authors discuss modern applications of flow cytometry, including the analysis of tumor cells, tumor infiltrating leukocytes, untouched isolation of tumor cells, exosome isolation and analysis, circulating tumor cells, and GMP-engineered T cells.
(Imprint: Nova)

Preface

Chapter 1. General Principles of Flow Cytometry
(David Aebisher, Sabina Galiniak, Tomasz Kubrak, Rafał Podgórski, Dorota Bartusik-Aebisher, Faculty of Medicine, University of Rzeszów, Poland)

Chapter 2. How Flow Cytometers Work
(Michał Osuchowski, David Aebisher, Piotr Przyczyna, Marek Biesiadecki, Piotr Bar and Dorota Bartusik-Aebisher, Faculty of Medicine, University of Rzeszów, Poland)

Chapter 3. Clinical Uses of Flow Cytometry
(Rafał Podgórski, Dominika Podgórska, David Aebisher, Sabina Galiniak, Tomasz Kubrak and Dorota Bartusik-Aebisher, Centre for Innovative Research in Medical and Natural Sciences, University of Rzeszów, Rzeszów, Poland, and others)

Chapter 4. Experimental Uses of Flow Cytometry
(Tomasz Kubrak, Rafał Podgórski, David Aebisher, Sabina Galiniak and Dorota Bartusik-Aebisher, Faculty of Medicine, Uniwersity of Rzeszów, Poland, and others)

Chapter 5. DNA Content Analysis by Flow Cytometry
(Dorota Bartusik-Aebisher, Sabina Galiniak, Tomasz Kubrak, Rafał Podgórski and David Aebisher, Faculty of Medicine, Uniwersity of Rzeszów, Poland)

Chapter 6. Flow Cytometery Data Analysis
(Marek Biesiadecki, David Aebisher, Piotr Bar, Michał Osuchowski, Piotr Przyczyna and Dorota Bartusik-Aebisher, Faculty of Medicine, University of Rzeszów, Poland)

Chapter 7. Flow Cytometry: Parameters and Probes
(Piotr Przyczyna, David Aebisher, Marek Biesiadecki, Piotr Bar, Michał Osuchowsk and Dorota Bartusik-Aebisher, Faculty of Medicine, University of Rzeszów, Poland)

Chapter 8. Flow Cytometry: Normal Blood and Bone Marrow Populations
(Natalia Leksa, David Aebisher, Joanna Gustalik, Elżbieta Ostańska and Dorota Bartusik-Aebisher, Faculty of Medicine, University of Rzeszów, Poland)

Chapter 9. Flow Cytometry: Acute Leukaemia
(Elżbieta Ostańska, David Aebisher, Joanna Gustalik, Natalia Leksa and Dorota Bartusik-Aebisher, Faculty of Medicine, Uniwersity of Rzeszów, Poland)

Chapter 10. Flow Cytometry: Disorders of Plasma Cells
(Joanna Gustalik, David Aebisher, Natalia Leksa, Elżbieta Ostańska and Dorota Bartusik-Aebisher, Faculty of Medicine, University of Rzeszów, Poland)

Chapter 11. The Crossmatch Assay in Solid Organ Transplantation
(Andrés Jaramillo, PhD, and Daniel S. Ramon, PhD, Department of Laboratory Medicine and Pathology
Mayo Clinic, Phoenix, AZ, US)

Chapter 12. Quantification of Soluble Molecules
(Sabina Galiniak, Tomasz Kubrak, Rafał Podgórski, David Aebisher and Dorota Bartusik-Aebisher, Department of Biochemistry, Faculty of Medicine, University of Rzeszów, Rzeszów, Poland)

Chapter 13. Flow Cytometers Future Alternative
(Piotr Bar, David Aebisher, Michał Osuchowski, Piotr Przyczyna, Marek Biesiadecki and Dorota Bartusik-Aebisher, Faculty of Medicine, Uniwersity of Rzeszów, Poland)

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