High Throughput Screening: Methods, Techniques and Applications

Mark Wigglesworth (Editor)
Director Hit Discovery, Global HTS, AstraZeneca, R&D, Innovative Medicines, Discovery Sciences, Mereside, Alderley Park, Macclesfield, Cheshire, United Kingdom

David Murray (Editor)
Associate Director, Hit Discovery, Discovery Sciences, IMED Biotech Unit, AstraZeneca, Alderley Park, UK

Geoffrey Holdgate (Editor)
Associate Director, Hit Discovery, Discovery Sciences, IMED Biotech Unit, AstraZeneca, Alderley Park, UK

Carolyn J. Blackett (Editor)
Associate Director, Hit Discovery, Discovery Sciences, IMED Biotech Unit, AstraZeneca, Alderley Park, UK

Series: Recent Trends in Biotechnology
BISAC: TEC059000

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High Throughput Screening (HTS) is one of several hit identification approaches that are part of a developing and evolving toolbox for the discovery of pharmaceutical start points. HTS remains one of the most successful approaches, and therefore an important foundation of drug discovery. In High Throughput Screening: Methods, Techniques and Applications, leading industrial and academic experts in screening and drug discovery explain key technologies and methods while demonstrating how they can be applied to successful hit identification. Describing both traditional and emerging methods in detail, this book provides an overview of these methods to the reader that will serve both those new to the field and expert scientists alike. High Throughput Screening: Methods, Techniques and Applications provides readers with an outline of key elements in the areas of assay development, detailed descriptions of a range of both biochemical and cell-based screening methodologies and strategies, as well as highlighting important steps in data analysis. By describing the basic principles of methods commonly used in HTS, High Throughput Screening: Methods, Techniques and Applications provides an illuminating introduction to HTS, capturing established good practice within the field, thereby imparting both the industrial and academic researcher with the knowledge required to work effectively in both today’s and the hit identification laboratories of the future.
(Imprint: Nova)

Preface

Chapter 1. Assay Development for High Throughput Screening
(David Cronk, Mohammed Akhlaq, Sachin Mahale and Joanne Shearer, Charles River Laboratories, Chesterford Research Park, Little Chesterford, Saffron Walden, UK)

Chapter 2. Application of Fluorescence Technologies to High Throughput Screening Assays
(Bolormaa Baljinnyam, Maya L. Gosztyla, Michael Ronzetti, Anton Simeonov and Nathan P. Coussens, National Center for Advancing Translational Sciences, National Institutes of Health, Rockville, MD, US)

Chapter 3. Luminescence Technologies for High Throughput Compound Screening
(Eun Jeong Cho and Kevin N. Dalby, College of Pharmacy, University of Texas at Austin, Austin, TX, US)

Chapter 4. Acoustic Mist Ionization Mass Spectrometry (AMI-MS) in High Throughput Screening
(Hannah Semple, Gareth Davies, Marian Preston, Corinne Molyneux, Charles-Hugues Lardeau, Elizabeth Mouchet and Ian Sinclair, Hit Discovery, Discovery Sciences, R&D, AstraZeneca, Macclesfield, Cheshire, UK, and others)

Chapter 5. High Throughput Flow Cytometry: Principles, Practice and Prospects
(Adam Zweifach, Department of Molecular and Cell Biology, University of Connecticut, Storrs, CT, US)

Chapter 6. Perspectives on High Content Imaging as an HTS Technology: Now and in the Future
(James Pilling, Samantha Peel, Elizabeth Mouchet and Guy Williams, Discovery Biology, Discovery Sciences, R&D, AstraZeneca, Cambridge, UK, and others)

Chapter 7. Biophysical Hit Evaluation
(Scott Pollack, Rupert Satchell, Juliet Morgan and Patrick J. McIntyre, Bioscience Department, Sygnature Discovery, BioCity, Nottingham, UK)

Chapter 8. CETSA in High Throughput Screening
(Joseph Shaw and Lorena A. Kallal, Discovery Biology, Discovery Sciences, R&D, AstraZeneca, Cambridge, UK, and others)

Chapter 9. Droplet-Based Microfluidics for High Throughput Screening
(Huiling Yuan, Ran Tu and Qinhong Wang, CAS Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China)

Chapter 10. Encoded Library Technologies as Screening Platforms for Drug Discovery
(Jonas V. Schaefer, Christoph E. Dumelin, Lukas Leder, and Johannes Ottl, Novartis Pharma AG/NIBR/CBT/Chemical Genetics/Encoded Library Technologies, Basel, Switzerland)

Chapter 11. Data Analysis in High Throughput Screening
(Stephan Steigele and Stephan Heyse, Genedata AG, Basel, Switzerland)

Chapter 12. The Application of Iterative Screening to Hit Identification
(Fredrik Svensson, Andreas Bender and Ulf Norinder, Alzheimer’s Research UK UCL Drug Discovery Institute, University College London, London, UK, and others)

About the Editors

Index

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