High-Performance Liquid Chromatography: Types, Parameters and Applications

Ivan Lucero (Editor)

Series: Analytical Chemistry and Microchemistry
BISAC: SCI013010




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In this collection, the authors discuss the way in which it is possible to detect the low concentrations at which toxic compounds and metabolites are present in specimens due to the huge development of chromatographic techniques. Chromatography is an analytical procedure used for the separation of compounds/drugs and its most commonly used types are based on either high performance liquid chromatography or gas chromatography, both coupled to mass spectrometry. As such, the advances in liquid chromatography coupled to different detectors, particularly mass spectrometry, giving examples of its applications in the areas of clinical and forensic toxicology. The authors present the importance of using high-performance liquid chromatography in analytical chemistry, as well as the positive aspects related to the use of chemometric approaches for the optimization of processes during the development of analytical methods. Case studies about real applications of chemometric tools focusing on the analysis of water samples polluted with a mixture of polycyclic aromatic hydrocarbons are presented. Next, sample preparation is considered as a difficult part of any analytical procedure in which the chemical compounds are present at low concentrations in complex matrices. Graphene based materials have been more and more popular in the procedure of sample preparation. Graphene is a material that has good physical-chemical properties, high fracture stretch, ultra-high specific surface area with nanosheets morphology and good mechanical and thermal stability. In the concluding study, the authors present a simple, economic and environmental friendly modification of stationary phase by self-assembly positive charged photosensitive diazoresin. The preparation and modification process of the chromatographic stationary phases is discussed. Additionally, the separation performance of chromatographic stationary phase is also discussed.


Chapter 1. The Role of Liquid Chromatography in Toxicological Analysis
(T. Rosado, S. Soares, S. Malaca, J. Gonçalves, M. Barroso, PhD, and E. Gallardo, PhD, Centro de Investigação em Ciências da Saúde, Universidade da Beira Interior (CICS-UBI), Covilhã, Portugal, and others)

Chapter 2. High-Performance Liquid Chromatography and Chemometric Techniques for Analytical Method Development
(Ainhoa Rubio-Clemente, Edwin L. Chica, and Gustavo A. Peñuela, Facultad de Ciencias de la Salud, Universidad Católica de Murcia UCAM, Murcia, Spain, and others)

Chapter 3. Graphene Based Materials in Sample Preparation Prior to HPLC Analysis and Their Applications
(Martha Maggira and Victoria F. Samanidou, Laboratory of Analytical Chemistry, Department of Chemistry, Aristotle University of Thessaloniki, Thessaloniki, Greece)

Chapter 4. Study on Preparation, Modification and Separation Performance of the Chromatographic Stationary Phase
(Bing Yu, Shuai Zhang, Fangyuan Lv, Yang Chen, Haonan Dong, and Hailin Cong, Institute of Biomedical Materials and Engineering, College of Chemistry and Chemical Engineering, Qingdao University, Qingdao, China, and others)


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