High-Performance Liquid Chromatography (HPLC): Principles, Practices and Procedures


Yuegang Zuo, PhD (Editor)
Department of Chemistry and Biochemistry, University of Massachusetts Dartmouth, North Dartmouth, MA, USA

Series: Chemical Engineering Methods and Technology
BISAC: TEC009010

High-performance liquid chromatography (HPLC) has emerged as the most powerful and versatile separation and analytical method. This book covers not only the conventional HPLC techniques but also the new developments, novel separation modes, column technology, as well as procedures and practices, particularly the advanced applications of HPLC in the fields of pharmaceutical, clinical, bioanalytical and food sciences. An in-depth discussion of separation mechanisms and characteristics of stationary and mobile phases is well-blended with practical method development and validation. Practical and pragmatic approaches and actual examples for rapid and effective development of fast, selective, accurate and reliable HPLC methods are provided.

This book consists of eleven chapters. The first chapter explains the basic principles of hydrophilic interaction liquid chromatography (HILIC), an appealing alternative HPLC separation mode for separating polar compounds along with the properties of HILIC stationary and mobile phases. The technical aspects and the applications as well as actual HILIC method development are illustrated. The second chapter describes recent progress in HPLC column technology. The advantages and disadvantages of sub-2 µm porous particles, solid core particles and monolithic columns over the conventional columns are discussed in terms of the separation efficiency, speed, resolution and applications. Chapters Three to Eleven are devoted to advanced applications of modern HPLC including RP-HPLC, NP-HPLC, Ion chromatography, HILIC, LC-MS/MS in pharmaceuticals, clinical, biomarkers, dietary supplements, food analysis and dye examination in archeological textiles. (Imprint: Nova)

Table of Contents

Table of Contents


Chapter 1. Hydrophilic Interaction Liquid Chromatography: Fundamentals and Applications
(Yuegang Zuo, Si Zhou, Ruiting Zuo, Tian Shi, Yang Yang and Patricia Henegan, Department of Chemistry and Biochemistry, University of Massachusetts Dartmouth, North Dartmouth, North Dartmouth, MA, USA and others)

Chapter 2. The New Generation of HPLC Columns: Evolution of Packing Materials
(Victoria Samanidou and Chrysa Nazyropoulou, Laboratory of Analytical Chemistry, Department of Chemistry, Aristotle University of Thessaloniki, Greece)

Chapter 3. High-Performance Liquid Chromatography for Carbohydrate Analysis
(Xun Yan, Analytical Sciences, Research & Development, Amway Corporation, Ada, MI, USA)

Chapter 4. High-Performance Liquid Chromatography for the Analysis of Non-Nucleoside Reverse Transcriptase Inhibitors in Biological Fluids and Pharmaceutical Products
(Virginia Melis, Iris Usach and José-Esteban Peris, Department of Pharmacy and Pharmaceutical Technology, Faculty of Pharmacy, University of Valencia, Avda. V. Andrés Estellés, Valencia, Spain)

Chapter 5. High-Performance Liquid Chromatography – An Effective Tool for Quality Control of Natural Cholesterol-Lowering Agents
(Ana Mornar, Miranda Sertić and Biljana Nigović, Faculty of Pharmacy and Biochemistry University of Zagreb, Zagreb, Croatia)

Chapter 6. Measurement of Cholesterol Levels of Lipoprotein Classes by Using Anion-Exchange Chromatography
(Yuji Hirowatari, Hidekatsu Yanai and Hiroshi Yohida, Bioscience Division, Tosoh Corporation, Japan and others)

Chapter 7. Recent Trends in LC Analysis of Biological Materials for Determination of Benzodiazepine Drugs
(Karolina Persona and Katarzyna Madej, Department of Analytical Chemistry, Faculty of Chemistry, Jagiellonian University, Ingardena 3 Street, 30-060 Krakow, Poland)

Chapter 8. HPLC Analytical Study of Methacrylic Monomers Released from Dental Composite Materials
(Giuseppina Nocca, Virginia Carbone, Alessandro Lupi, Sandro Rengo and Gianrico Spagnuolo, Istituto di Biochimica e Biochimica Clinica, Università Cattolica del Sacro Cuore, Roma, Italy and others)

Chapter 9. HPLC Analysis of Vitamin B1, B2, B3, B6, B9, B12 and Vitamin C in Various Food Matrices
(Jessy van Wyk, Larry Dolley and Ndumiso Mshicileli, Department of Food Technology, and Agrifood Technology Station, Cape Peninsula University of Technology, Bellville, South Africa)

Chapter 10. Ultra Performance Liquid Chromatography Coupled to Ultraviolet-Vis and Mass Spectrometry Detector for Screening of Organic Acids and Polyphenols in Red Wine
(M. Gonzalez-Hernandez, J. M. Avizcuri-Inac, M. Dizy and P. Fernandez-Zurbano, Departamento de Agricultura y Alimentación, Madre de Dios 51, 26006 Logroño, La Rioja, Spain and others)

Chapter 11. HPLC–UV-Vis–ESI MS Examination of Archeological Fibers – Red Natural Dyes in Italian Textiles from 15th and 16th Centuries
(Katarzyna Lech and Maciej Jarosz, Warsaw University of Technology, Faculty of Chemistry, Chair of Analytical Chemistry, Warsaw, Poland)


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