Handbook on Protein Purification: Industry Challenges and Technological Developments

Nikolaos E. Labrou, Ph.D. (Editor)
Agricultural University of Athens, Athens, Greece

Evangelia G. Chronopoulou, Ph.D. (Editor)
Agricultural University of Athens, Greece

Farid Ataya, Ph.D. (Editor)
King Saud University, Riyadh, Saudi Arabia (KSA)

Series: Biochemistry Research Trends
BISAC: SCI007000

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Proteins are important biomolecules that are vital for the cellular structure and function. They perform a vast array of functions within organisms, including the catalysis of metabolic reactions, DNA replication, response to stimuli, and transporting molecules from one location to another. The technological advances in the omics areas (e.g., genomics, transcriptomics, proteomics, metagenomics, etc.) have dramatically increased the rate of discovering new proteins. Some of them hold large opportunities for innovative research and the development of commercial products and applications. It is worth noting that the global protein ingredients market is poised to grow over the next decade to reach approximately 58.49 billion USD by 2022, with the protein therapeutics market valuing around USD 315.9 billion by 2025. Interestingly, about seventy monoclonal antibody products will be on the market by 2020 with a combined worldwide sales of about 125 billion USD.

The most significant parameter for the successful commercial exploitation of proteins rely on the development of an efficient and effective isolation and purification technology, known as protein downstream processing. Downstream processing refers to the technology that involves the isolation and production of purified products from natural sources such as animal tissues, plant tissues, microorganisms or fermentation broth. The most important element of this technology is the high purification process, most importantly of which is chromatography and in particular affinity chromatography.

The present book would definitely be an ideal source of scientific information to the advanced students, junior researchers, and scientists involved in cellular and molecular biology, biochemistry, microbiology, biotechnology and other related areas.

Preface

Chapter 1. Column Chromatography Overview: An Evolving Method in Protein Purification
(Evangelia G. Chronopoulou, Laboratory of Enzyme Technology, Department of Biotechnology, School of Food, Biotechnology and Development, Agricultural University of Athens)

Chapter 2. Design, Synthesis and Applications of Short-Peptide and Peptidomimetic Ligands for Affinity Chromatography
(Soledad L. Saavedra, Gabriela R. Barredo, María C. Martínez-Ceron, Silvana L. Giudicessi, Osvaldo Cascone, Fernando Albericio and Silvia A. Camperi, Cátedra de Biotecnología. Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires. Buenos Aires, Argentina, and others)

Chapter 3. Design, Synthesis and Application of a Synthetic Biomimetic Ligand for the Affinity Purification of Therapeutic Proteins
(Dimitris Platis and Nikolaos E. Labrou, Laboratory of Enzyme Technology, Department of Biotechnology, School of Food, Biotechnology and Development, Agricultural University of Athens, Athens, Greece)

Chapter 4. Comparative Analysis of the Equilibrium Binding and Adsorption Kinetics of Cho- and Maize-Derived Recombinant Monoclonal Antibodies on Protein A Affinity Adsorbent
(Dimitris Platis, Anastasios Maltezos, Evangelia G. Chronopoulou, Farid Ataya and N.E. Labrou, Laboratory of Enzyme Technology, Department of Biotechnology, School of Food, Biotechnology and Development, Agricultural University of Athens, Athens, Greece, and others)
Chapter 5. Protein Purification from Protein Aggregates
(Neus Ferrer-Miralles, Paolo Saccardo and Elena Garcia-Fruitós, Institut de Biotecnologia i de Biomedicina, Universitat Autònoma de Barcelona, Bellaterra, Cerdanyola del Vallès, Spain, and others)

Chapter 6. Challenges in Dairy Whey Protein Purification
(Nicolás Urtasun, María Fernanda Baieli, Daniela Hirsch, María Victoria Miranda, Osvaldo Cascone and Federico Javier Wolman, Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Cátedra de Biotecnología. Buenos Aires, Argentina, and others)

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