Genome Editing for Crop Improvement


Rahul Kumar, PhD – Associate Professor, Faculty of Agricultural Sciences, DAV University, Jalandhar, Punjab, India
Ashutosh Sharma, MSc, MPhil, PhD, PGDAEM – Associate Professor, Faculty of Agricultural Sciences, DAV University, Jalandhar, India (ORCID: 0000-0003-3101-0116)
Yogesh Kumar, PhD – Associate Professor, Department of Botany, Central University of Jammu, J&K, India
Indu Sharma, MSc, PhD – Associate Professor, Department of Life Sciences, Sant Baba Bhag Singh University, Jalandhar, India (ORCID: 0000-0003-0302-9370)

Series: Agriculture Issues and Policies
BISAC: TEC003030

Over the last few decades, the agricultural land is decreasing continuously with concurrent rise in human population. Therefore, ensuring food security for all is a major challenge. Therefore, to assist the classical plant improvement interventions, there is an urgent need to develop new methods to improve the crops in a faster, ecologically safer and in a more precise way. There has been a fairly large number of tools available for the targeting and precise editing of plant genomes.

In this regard several different technologies, particularly the zinc fingers (ZFNs), transcription activator-like effector nucleases (TALENs), and clustered regularly interspaced short palindromic repeat (CRISPR) have been shown to have a high potential to precisely edit the plant genome to improve crop plants. Keeping this in mind the present book was designed to cover all major areas of plant genome editing covering the discovery, types, regulations/policies, challenges, opportunities, and ethical issues associated with genome editing; besides covering more technical parts like bioinformatics tools for genome editing, techniques, off-targeting etc. Moreover, it also covers the editing the plant genomes for some particular traits (resistance against plant pathogens, parasitic weeds or a particular types of stress) or crop types (legumes, model plants, cereals and horticultural crops etc.). Overall, this is book is a wholesome package on genome editing for crop improvement in the form of 16 state-of-the-art edited chapters, written by different researchers/academicians to present up-to-date information on the subject. This book is an excellent study material for the students and the researchers interested to work in the field of genome editing.

Table of Contents


Chapter 1. Genome Editing: Opportunities and Challenges
Harsharan Singh
PG Department of Biotechnology, Lyallpur Khalsa College, Jalandhar, Punjab, India

Chapter 2. Plant Genome Editing Using the CRISPR System
Satyananda Patel¹ and Rahul Kumar²
¹MITS School of Biotechnology, Bhubaneswar, Odisha, India
²Faculty of Agricultural Sciences, DAV University, Jalandhar, Punjab, India

Chapter 3. Recent Progression in New Approaches and Technologies in Plants Using the CRISPR/Cas9 System for Genome Editing
Pooja Kumari, Preeti Sharma, Poornima Pandey and Narendra Kumar Sharma
Department of Bioscience and Biotechnology, Banasthali Vidyapith, Tonk Rajasthan, India

Chapter 4. A DNA-Free Genome Editing System
Anuj Sharma and Karan Paul
Department of Biochemistry, DAV University, Jalandhar, India

Chapter 5. Experimental and Bioinformatics Approaches to Predict Off-Targeting of Programmable Nucleases (ZFNs, TALENs and CRISPR-Cas9)
Purnima Sharma¹, Harpreet Singh¹, Rupinder Preet Kaur², Kamini¹, Rakhi¹, Taniya¹ and Nandni Kanwar¹
¹PG Department of Bioinformatics, Hans Raj Mahila Maha Vidayala, Jalandhar, Punjab, India
²Department of Chemistry, Guru Nanak Dev University College Verka, Amritsar, Punjab, India

Chapter 6. Regulations and Policies of Genome Editing in Plants
Sukhdeep Kumar
Assistant Professor, Department of Medical Laboratory Sciences, School of Allied Medical Sciences, Lovely Professional University, Phagwara, Punjab, India

Chapter 7. CRISPR-Cas-Mediated Genome Editing Strategies for Abiotic Stress Tolerance in Crops
Meghna Patial¹, Ram Kumar Sharma¹,² and Rohit Joshi¹,²
¹Division of Biotechnology, CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh, India
²Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, Uttar Pradesh, India

Chapter 8. CRISPR/Cas: A Promising Implement for Empowering Plants against Viral Stress
Asha Rani¹, Rashmi Suman¹, Taruna Gupta¹, Narayan Rishi¹, Yashika Walia², Sunny Dhir² and Vanita Chandel¹
¹Amity Institute of Virology and Immunology, Amity University Uttar Pradesh, Noida U.P., India
²Department of Biotechnology, Maharishi Markandeshwar (Deemed to be University), Mullana, Ambala [Haryana], India

Chapter 9. CRISPR-Cas9-Based Genome Editing for Disease Resistant Plants
Poornima Pandey, Mansi Srivastava, Preeti Sharma, Narendra Kumar Sharma and Pracheta Janmeda
Department of Bioscience and Biotechnology, Banasthali Vidyapith, Tonk, Rajasthan, India

Chapter 10. A Genome Editing Approach to Enhance Host Resistance against Plant-Parasitic Weeds
Vinay Kumar Bari
Department of Biochemistry, School of Basic Sciences, Central University of Punjab, VPO-Ghudda, Bathinda, Punjab, India

Chapter 11. CRISPR-Mediated Genome Editing in Model Plants
Vinita Sharma¹, Ashita Bisht²,³, Ankita Mishra¹, Himanshu Sharma¹, Joy Roy¹ and Abhishek Bhandawat¹
¹National Agri-Food Biotechnology Institute, Mohali, Punjab, India
²Department of Plant Breeding and Genetics, Punjab Agriculturale University, Ludhiana, Punjab, India
³Department of Genetics and Plant Breeding, CSKHPKV, Palampur, H. P., IndiaCSK Himachal Pradesh Krishi Vishvavidyalaya, Highland Agricultural Research and Extension Centre, Kukumseri, Lahaul and Spiti, India

Chapter 12. Genome Editing in Some Cereal Crops
Priya Chaudhary¹, Pracheta Janmeda¹, Poornima Pandey¹, Devendra Singh² and Rahul Kumar³
¹Department of Bioscience and Biotechnology, Banasthali Vidyapith, Rajasthan, India
²Department of Chemistry, Mohanlal Sukhadia University, Udaipur, Rajasthan, India
³Faculty of Agricultural Sciences, DAV University, Jalandhar, Punjab, India

Chapter 13. Genome Editing in Horticultural Crops
Aditi Thakur¹, Suman Sanju², Reena Kumari¹, Karuna Dhiman¹ and Sneh Sharma¹
¹College of Horticulture and Forestry, Neri, Hamirpur, (H. P), Dr. Yashwant Singh Parmar University of Horticulture and Forestry, Nauni, Solan (H. P), India
²Department of Botany, Abhilashi University, Mandi (H. P), India

Chapter 14. Genome Editing in Vegetables and Fruit Crops: Present Status and Future Prospects
Baljeet Singh¹,², Urvashi Mittal¹, Sarvjeet Kukreja³, Vijay Kumar¹, Harmeet Kaur¹, Vinay Bhardwaj², Sundaresha Sidappa² and Umesh Goutam¹,⁴
¹Department of Biotechnology, School of Bioengineering and Biosciences, Lovely Professional University, Punjab, India
²ICAR-Central Potato Research Institute, Shimla
³Department of Agronomy, School of Agriculture, Lovely Professional University, Punjab
⁴Department of Molecular Biology and Genetic Engineering, School of Bioengineering and Biosciences, Lovely Professional University, Punjab, India

Chapter 15. Prospects of CRISPR/Cas9-Mediated Gene Editing in Legume Crops
Anil Kumar Singh
Department of Agriculture, Sant Baba Bhag Singh University, Khiala, Punjab, India

Chapter 16. Genome Editing in Grain and Fodder Legumes: Current Status and Opportunities
Neha Sharma¹ and Himani Sharma²
¹CSK HPKV HAREC Bajaura, Kullu, India
²Dr. YS Parmar COH&F, Neri, Hamirpur, India


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