Gene Delivery: Methods and Applications

Vanessa Zimmer (Editor)

Series: Biochemistry and Molecular Biology in the Post Genomic Era
BISAC: SCI029000

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Volume 10

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Special issue: Resilience in breaking the cycle of children’s environmental health disparities
Edited by I Leslie Rubin, Robert J Geller, Abby Mutic, Benjamin A Gitterman, Nathan Mutic, Wayne Garfinkel, Claire D Coles, Kurt Martinuzzi, and Joav Merrick

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Gene Delivery: Methods and Applications provides a comprehensive overview on viral and non-viral methods used to genetically engineer human mesenchymal stromal cells. In addition, an update on ongoing and completed clinical studies with engineered mesenchymal stromal cells will be provided, as well as a snapshot of the advances and technical challenges yet to be addressed.

Next, a variety of gene delivery systems including physical transfection techniques, virus-based delivery vectors, chemically engineered delivery systems and bio-inspired vehicles are reviewed and their strengths, shortcomings and biomedical applications are discussed.
Selfish DNA called transposons capable of cutting out and pasting into the host genome are active throughout the phylogenetic kingdoms. Researchers have repurposed natural transposons for use in delivering a gene-of-interest, enabling for the study of a large and growing list of preclinical gene therapy applications. As such, the authors discuss the past achievements and future challenges of this early-stage technology.

The closing chapter introduces cell-penetrating peptides as an efficient tool for DNA transfection. HR9, a designed cell-penetrating peptides, containing nona-arginine flanked by cysteine and penta-histidine displayed a high penetrating ability in mammalian cells.
(Imprint: Nova)

Preface

Chapter 1. Gene Delivery as a Tool to Improve Therapeutic Features of Mesenchymal Stromal Cells: Methods and Applications
(Marília Silva, Cristiana Ulpiano, Nuno Bernardes, Gabriel A. Monteiro and Cláudia Lobato da Silva, Department of Bioengineering and iBB-Institute for Bioengineering and Biosciences, Instituto Superior Técnico, Universidade de Lisboa, Lisboa, Portugal, and others)

Chapter 2. Nucleic Acid Delivery Systems for Gene Therapy
(Jinhyung Lee and Yupeng Chen, Department of Biomedical Engineering, University of Connecticut, Storrs, CT, US)

Chapter 3. Targeting Transposons to the Genome
(Jesse B. Owens, Institute for Biogenesis Research, Anatomy, Biochemistry and Physiology, John A. Burns School of Medicine, University of Hawaii at Manoa, Honolulu, Hawaii, US)

Chapter 4. Efficient DNA Transfection in Protists mediated by Cell-Penetrating Peptide
(Betty Revon Liu, Yue-Wern Huang and Han-Jung Lee, Department of Laboratory Medicine and Biotechnology, Tzu Chi University, Hualien, Taiwan, and others)

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