Gel Electrophoresis: Types, Applications and Research

Gilbert H. Mitchell (Editor)

Series: Biochemistry Research Trends
BISAC: SCI007000

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Polyacrylamide gel electrophoresis (PAGE) is performed routinely in research and diagnostics. The lack of internal standards, however, has been very disadvantageous for PAGE methodologies in comparison to alternative separation techniques like chromatography. Chapter one discusses how comparative fluorescence gel electrophoresis (CoFGE) has the potential to change this situation. Chapter Two focuses on elucidating the properties of polyacrylamide gel matrixes, discussing the essential principles of discontinuous PAGE in technology, and summarizing the discontinuous buffer systems employed for the discontinuous PAGE in alkaline, neutral and acidic circumstances. Chapter Three discusses the need for the establishment of typical physiologic protein pattern for various avian patients. Chapter Four discusses constant field gel electrophoresis, a sensitive, rapid, economical and perspective method for detecting DNA double strand breaks and rejoining with high resolution. (Imprint: Nova)

Preface

Chapter 1. Introducing Standardization to Gel Electrophoresis by Comparative Fluorescence Gel Electrophoresis (CoFGE0
Simone König (Core Unit Proteomics, Interdisciplinary Center for Clinical Research, Westfalian Wilhelms-University of Münster, Münster, Germany)

Chapter 2. Discontinuous Polyacrylamide Gel Electrophoresis
Li Sun, Mingri Zhao, Shumei Wang, Haiping Diao and Shengnan Wang (College of Life Sciences, Yantai University, Yantai, Shandong, P. R. China, and others)

Chapter 3. Protein Electrophoresis in Avian Medicine
Csilla Tóthová, Peter Major, Ladislav Molnár and Oskar Nagy (Clinic of Ruminants, University of Veterinary Medicine and Pharmacy, Košice, Slovak Republic, and others)

Chapter 4. Constant Field Gel Electrophoresis: Sensitive Method for DSBs Detection
Svetla P. Gateva, PhD and Stephka G. Chankova, PhD (Department of Ecosystem Research, Environmental Risk Assessment and Conservation Biology, Institute of Biodiversity and Ecosystem Research, BAS, Sofia, Bulgaria)

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