Flow Cytometry: Principles, Methodology and Applications

Stefanos Papandreou (Editor)

Series: Cell Biology Research Progress
BISAC: SCI017000

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$140.00

Volume 10

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Special issue: Resilience in breaking the cycle of children’s environmental health disparities
Edited by I Leslie Rubin, Robert J Geller, Abby Mutic, Benjamin A Gitterman, Nathan Mutic, Wayne Garfinkel, Claire D Coles, Kurt Martinuzzi, and Joav Merrick

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Flow cytometry is a method to conduct a multiparameter analysis of cells suspended in liquid and passing through a laser beam. As cells pass through the flow chamber, they are hit with a laser light beam which is scattered in different directions, and recorded as forward light-scatter and side light-scatter. In this book, the authors discuss the principles, methodology and applications of flow cytometry.

Topics include flow cytometry and epifluorescence analyses of freshwater bacterio- and virioplankton communities; application of flow cytometry in phenotyping, analysis and functional characterization of T regulatory and immune suppressor cells; multicolor panel design strategies in rare event analysis of dendritic cells and regulatory T cells; advanced characterization of individual particle morphology light-scattering flow cytometry; and application of flow cytometry in environmental science. (Imprint: Nova Biomedical )

Preface pp.vii-ix

A Few Tests Prior to Flow Cytometry and Epifluorescence Analyses of Freshwater Bacterio- and Virioplankton Communities
(Stéphan Jacquet, Ursula Dorigo, Sébastien Personnic, INRA, UMR CARRTEL, Thonon cedex, France)pp.1-30

Application of Flow Cytometry in Phenotyping, Analysis and Functional Characterization of T Regulatory and Immune Suppressor Cells: A Focus on Multiple Myeloma Immune Regulatory Biology
(Karthick Raja Muthu Raja, Roman Hajek, Babak Myeloma Group, Department of Pathological Physiology, Faculty of Medicine, Brno, Czech Republic, and others)pp.31-56

Multicolor Panel Design Strategies in Rare Event Analysis: Dendritic Cells and Regulatory T Cells
(Ilse Van Brussel, Nathalie Ruyssers, Marthe Heylen, Dorien M. Schrijvers, University of Antwerp, Laboratory of Physiopharmacology and Gastroenterology, Universiteitsplein, Antwerp, Belgium)pp.57-78

Light-Scattering Flow Cytometry: Advanced Characterization of Individual Particle Morphology
(Valeri P. Maltsev, Andrey V. Chernyshev, Dmitry I. Strokotov, Institute of Chemical Kinetics and Combustion, Novosibirsk, Russian Federation, and others)pp.79-104

Applications of Flow Cytometry in Environmental Sciences: Inspiring Examples
(Piotr Rzymski, Barbara Poniedzia³ek, Department of Biology and Environmental Protection, Poznan University of Medical Sciences, D³uga, Poznañ, Poland)pp.105-112

Index pp.113-120

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