Exploring the Biotechnological Potential of C2 Alternative Substrates in Biocatalysis and Metabolic Engineering

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Wenfa Ng (Author) – Department of Biomedical Engineering, National University of Singapore, Singapore

Series: Recent Trends in Biotechnology
BISAC: SCI010000
DOI: https://doi.org/10.52305/GTIA8095

Metabolic engineering and biocatalysis aim to produce new value-added products from biological routes through careful and deliberate genetic engineering in different microbial chassis with suitable metabolic repertoire. Hitherto, the endeavor has largely been confined to the production of new products from glucose and a few other well characterized substrates. But, as the bioeconomy gains in importance and the world seeks to explore new sources of carbon as feedstocks, a possibility exists in developing alternative C2 compounds as feedstocks in contrast to the current reliance on C6 (glucose and fructose), C5 (xylose) and C3 (glycerol) substrates. Two potential C2 alternative substrates explored in this work are ethanol and ethylene glycol. Ethanol is a biofuel whose biotechnological potential has not been fully explored. In the first part of the book, the potential of using the NADH generated from a reconstructed ethanol utilization pathway to power a biotransformation reaction is explored in Escherichia coli. Experimental results indicate that ethanol utilization delivers higher biotransformation efficiency compared to state-of-the-art glucose dehydrogenase system, which confirmed theoretical predictions of relative efficiency of the two systems. Next, the book switches attention to ethylene glycol which could be derived as breakdown products of lignocellulosic waste or from plastic waste. Here, the proposal is to demonstrate the potential of an NADH generating ethylene glycol utilization pathway that terminates at glycolate to support biocatalytic reactions in E. coli. Subsequently, the two-step ethylene glycol utilization pathway would be extended to malate and 2-phosphoglycerate to help another biotechnology workhorse, Bacillus subtilis, to grow on ethylene glycol as the sole carbon source. Different connection points to central carbon metabolism would provide a natural experiment to decipher the relative efficiencies of activating tricarboxylic acid cycle and glycolysis in supporting cell growth, and illuminate insights into the relative efficiency and evolutionary importance of different branches of metabolism in B. subtilis. Finally, ethylene glycol utilization would be combined with product formation to help B. subtilis to synthesize green fluorescent protein using flux from the C2 alternative substrate. This system would provide a platform for examining the efficacy of different ethylene glycol utilization pathways and entry points in central carbon metabolism in synthesizing amino acids and proteins. Overall, this book hopes to explore the biotechnological potential of two alternative C2 substrates (ethanol and ethylene glycol) in biocatalysis and metabolic engineering from the substrate utilization perspective.

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Table of Contents

Preface

Acknowledgments

Chapter 1. Introduction

Chapter 2. Developing Ethanol Utilisation for NADH Regeneration in Biocatalysis

Chapter 3. Future Project A: Use of Ethylene Glycol Utilization Pathway for Regenerating NADH in Escherichia Coli

Chapter 4. Future Project B: Teaching Bacillus Subtillis to Grow on the Unconventional Substrate Ethylene Glycol through Metabolic Engineering

Chapter 5. Future Project C: Evaluating if Connecting Ethylene Glycol to Acetyl-Coa in Bacillus Subtilis Is the Optimal Approach for Powering up Amino Acid and Protein
Synthesis

Chapter 6. Conclusion

References

Index

Additional information

Binding

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