ELISA: History, Types and Applications

Md Rezaul Karim (Editor)
Taihe Hospital of Hubei University of Medicine, Shiyan, Hubei, P.R. China

Series: Medical Procedures, Testing and Technology
BISAC: SCI007000

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ELISA: History, Types and Applications contains unique combinations of chapters in which the abstract of each chapter defines the work considered for the entire chapter. One of the essential goals of this book is to delineate vital information about different types of ELISA and their applications. The beginning chapter describes the history and background of enzyme-linked immunosorbent assay (ELISA), as well as the remarkable changes that were brought about by this technique. The chapter also describes the commercial application techniques used in food and diagnostic industries. The second chapter, on the other hand, describes various types of ELISA and their steps to perform ELISA. The third chapter discusses the practical applications and their mechanisms from theory. It also discusses possible multiple sources of error which can exist at each stage of the process; how to minimize the error; and the principles of data analysis. The fourth chapter discusses the applications of ELISA in various fields, which also include the uses of ELISA in the diagnosis of infectious diseases, parasitic diseases, and non-communicable disease caused by a variety of pathogenic microorganisms. While Chapters One to Four describe a detailed history, a variety of ELISA, their steps, their sources of application error, their solutions and their applications in different fields, the remaining chapters discuss more specific issues, including: The role of ELISA in advanced glycerin end products and their cell surface receptors in metabolic diseases, indirect ELISA for the diagnosis of Brucella Canis, ELISA based detection in cancer research, etc.

Preface

Acknowledgements

Chapter 1. The History and Background of Enzyme-Linked Immunosorbent Assay
(Olusola Olayiwola, Department of Biological Sciences, Ajayi Crowther University, Oyo, Oyo State, Nigeria)

Chapter 2. Types of Enzyme-Linked Immunosorbent Assays
(Yu-jie Yang, Md R. Karim and Yun-fu Wang, Department of Neurology, Renmin Hospital of Wuhan University, Wuhan, Hubei, China, and others)

Chapter 3. ELISA: From Theory to Practice
(Karishma Shah, Anindyt Nagar and Panayiotis Maghsoudlou, Nuffield Department of Orthopaedics, Rheumatology and Musculoskeletal Sciences, University of Oxford, Oxford, UK, and others)

Chapter 4. Application of Enzyme-Linked Immunosorbent Assay
(Yu-jie Yang, Md R. Karim and Yun-fu Wang, Department of Neurology, Renmin Hospital of Wuhan University, Wuhan, Hubei, China, and others)

Chapter 5. Enzyme-Linked Immunoassay, Advanced Glycation End Products, and Their Cell Surface Receptor in Metabolic Diseases
(Armando Gomez-Ojeda, Claudia Luevano-Contreras, Ma E. Garay-Sevilla, Katarzyna Wrobel and Kazimierz Wrobel, Department of Medical Sciences, Health Science Division, University of Guanajuato, Guanajuato, Mexico, and others)

Chapter 6. The Development of an Indirect ELISA Test for the Diagnosis of Brucella canis
(Zeki Aras, Department of Microbiology, Faculty of Veterinary Medicine, Aksaray University, Aksaray, Turkey)

Chapter 7. The Key Role of Immunoassay Based Detection in Cancer Research
(Armando Gomez-Ojeda, Yeniley Ruiz-Noa and Lorena D.R. Ibarra-Reynoso, Department of Medical Sciences, Health Science Division, University of Guanajuato, Guanajuato, Mexico)

Chapter 8. Enzyme-Linked Immunosorbent Assay for the Detection of Helicobacter Pylori
(Afsarunnesa Syeda, Department of Gynaecology & Obstetrics, Renmin Hospital of Hubei University of Medicine, Shiyan, Hubei, China)

Index

Keywords: ELISA, History of ELISA, Types of ELISA, Application of ELISA, Cancer Research, Metabolic Diseases, Brucella Canis, Helicobacter Pylori

Audience: Researchers, Pathologists, Toxicologists, ELISA Kit agents, Individuals working on ELISA research and development, Laboratory Technicians in medical laboratories for the in vitro diagnostics and serological blood tests, Teachers of ELISA laboratory course, Graduate students who require ELISA for their research, Applicants in the food industry in detecting potential food allergens, etc.

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