ELISA: Advances in Research and Applications

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Jose Ma. M. Angeles, PhD (Editor) – Department of Parasitology, College of Public Health, University of the Philippines, Manila, Philippines

Series: Medical Procedures, Testing and Technology
BISAC: MED018000
DOI: https://doi.org/10.52305/NIRE6124

In September 1971, Swiss scientists Eva Engvall and Peter Perlmann published a communication in the journal Immunochemistry on enzyme-linked immunosorbent assay (ELISA) as a quantitative assay of immunoglobulin G. Fifty years later, ELISA is still the most commonly used technique in measuring peptides, proteins, antibodies, antigens, and hormones in various samples. This plate-based assay is a very versatile laboratory technique and offers many advantages to laboratory scientists and researchers such as generating quick results, simple protocols, high sensitivity, being easily automated and being convenient to perform. Its application in the diagnosis of different infectious diseases has shown the unquestionable importance of this technique even in the modern world.

In this book, the usefulness of ELISA in the control of different parasitic diseases including schistosomiasis, babesiosis, and malaria are discussed. There is also a chapter featuring an extensive discussion on the use of ELISA in functional food development and regulation. And finally, the last two chapters will present advanced techniques inspired by ELISA including a rapid immunoassay for detecting paralytic shellfish poisoning toxins and the enzyme-linked aptamer assay. The Editor hopes this book will provide significant information regarding the current advances and applications of ELISA and possibly inspire its use in another 50 years.

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Table of Contents

Preface

Chapter 1. Updates in the Diagnostic Use of ELISA for Schistosomiasis
(Kevin Austin L. Ona and Jose Ma. M. Angeles – College of Medicine, University of the Philippines Manila, Manila, Philippines, et al.)

Chapter 2. Use of an ELISA to Assess the Immunogenicity and Protective Potential of a Babesia Bigemina RAP-1, GP45, and 12D3 Recombinant Protein Cocktail in Cattle
(Rebeca M. Santamaria-Espinosa, José J. Lira-Amaya, Patricia Vargas-Urióstegui, Roberto O. Castañeda-Arriola, Grecia Martínez-García, J. Antonio Álvarez-Martínez, Carmen Rojas-Martínez and Julio V. Figueroa-Millán – Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad, Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias Jiutepec, Morelos, México)

Chapter 3. ELISA Test Using Babesia Sp. Recombinant Antigens for Epidemiological Diagnosis of Bovine Babesiosis in Mexico
(Julio V. Figueroa-Millán, Roberto O. Castañeda-Arriola, José J. Lira-Amaya, Patricia Vargas-Urióstegui, J. Antonio Álvarez-Martínez and Carmen Rojas-Martínez – Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad, Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias Jiutepec, Morelos, México)

Chapter 4. ELISA: A Sero-Surveillance Tool in Malaria Elimination
(Pilarita T. Rivera – Department of Parasitology, College of Public Health, University of the Philippines Manila)

Chapter 5. Applications of Enzyme-Linked Immunosorbent Assays in Functional Food Development and Regulation
(Deborah S. Nicdao, Princess Alyssa S. Abid, Mia Beatriz C. Amoranto, Joseph Q. Bechayda, Sharah Kristine G. Bonguit, Calline Danica D. Gomez and Marilen Parungao Balolong – Applied Microbiology for Health and Environment Research Group (AMHERG), University of the Philippines Manila, Padre Faura Street, Ermita, Manila, Philippines, et al.)

Chapter 6. A Refined Rapid Antibody-Based Immunoassay Improved Sensitivity of Paralytic Shellfish Poisoning Toxins Detection
(Chun-Kwan Wong, Pui-Yi Cheung and Rickjason Chi-Wai Chan – Public Health Laboratory Services Branch, Centre for Health Protection, Department of Health, Hong Kong, China)

Chapter 7. Aptamers: The Chemical Antibodies, Their Emergence, and Application to Enzyme-Linked Immunosorbent Assay
(Altair B. Agmata, Chembie A. Almazar, Marjo V. Mendoza and Windell L. Rivera – Pathogen-Host-Environment Interactions Research Laboratory, Institute of Biology, College of Science, University of the Philippines Diliman, Quezon City, Philippines)

Index

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