Combined Use of Biopolymer Chitosan and Enzyme Tyrosinase for Removal of Bisphenol A and its Derivatives from Aqueous Solutions
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Kazunori Yamada, Mizuho Suzuki, Ayumi Kashiwada and Kiyomi Matsuda
Nihon University, Chiba, Japan, and others
Series: Biotechnology in Agriculture, Industry and Medicine
BISAC: SCI010000
This book investigates the availability of chitosan for removal of bisphenol A (BPA, 2,2-bis(hydroxyphenyl)propane) through the tyrosinase-catalyzed quinone oxidation. First, the process parameters, such as the hydrogen peroxide (H2O2)-to-BPA ratio, pH value, temperature, and tyrosinase dose, were discussed in detail for the enzymatic quinone oxidation of BPA. Tyrosinase-catalyzed quinone oxidation of BPA was found to be effectively enhanced by adding H2O2, and the optimum conditions for BPA at 0.3 mM were determined to be pH 7.0 and 40ºC in the presence of H2O2 at 0.3 mM ([H2O2]/[BPA]=1.0). Removal of BPA from aqueous solutions was accomplished by adsorption of enzymatically generated quinone derivatives on chitosan beads. (Imprint: Nova Biomedical)