Clinical Flow Cytometry: Approaches, Principles, and Applications

Jason Howard Kurzer, M.D., Ph.D. (Editor)
Director of Clinical Hematology Laboratory, Department of Pathology, Stanford University School of Medicine, Stanford, CA, US

Olga Kagan Weinberg, M.D. (Editor)
Director of Hematopathology and Flow Cytometry, Department of Pathology, Boston Children’s Hospital, Boston, MA, US

Series: Pathology and Laboratory Medicine
BISAC: MED067000

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This book is a focused review of clinical flow cytometry, and is meant to be helpful in daily clinical practice for those just beginning to learn flow cytometry as well as those with years of experience. Covering the basic principles of flow cytometry, and then engaging in detailed reviews of the flow cytometric evaluation of B-cells, plasma cells, T-cells, and myeloid cells, it packs a wide variety of immunophenotypic data into one volume. Moreover, this book covers both normal and abnormal findings for each lineage, and highlights key pitfalls to avoid making diagnostic mistakes. Many of the most common neoplastic entities are reviewed, and signature findings are highlighted. Using the updated nomenclature for clinical hematologic malignancies provided by the revised 4th edition of the WHO classification system, the book is current in its approach and content. Whenever possible, detailed colored examples of flow cytometric plots are provided to help convey the important diagnostic findings. Most importantly, a review of current applications of flow cytometry in minimal residual disease is provided to assist in both the development and interpretation of these assays. Written by experts in the field, the result is a practical resource for use as an everyday clinical reference.

Preface

Chapter 1. Introduction to Flow Cytometry
(Jason H. Kurzer, M.D., Ph.D., and Olga K. Weinberg, M.D., Department of Pathology, Stanford University School of Medicine, Stanford, CA, US)

Chapter 2. Introduction to Flow Cytometric Immunophenotyping of Benign B-Cells
(Teresa A. Scordino, M.D., James P. Harl and Sandra Admire, Department of Pathology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, US)

Chapter 3. Immunophenotyping of Normal and Abnormal Precursor B-cells
(Karen M. Chisholm, M.D., Ph.D., Department of Pathology, Seattle Children’s Hospital, University of Washington, Seattle, WA, US)

Chapter 4. Mature B-Cell Neoplasms
(Daniel F. Boyer, M.D, Ph.D., Department of Pathology, University of Michigan, Ann Arbor, Michigan)

Chapter 5. Flow Cytometry Immunophenotyping of Plasma Cells & Plasma Cell Neoplasms
(Andrew G. Evans, M.D., Ph.D., Department of Pathology and Laboratory Medicine, University of Rochester, Rochester, NY, US)

Chapter 6. Flow Cytometric Immunophenotyping of Benign Reactive T-Cells
(Jean S. Oak, M.D., Ph.D., Department of Pathology, Stanford University School of Medicine, Stanford, CA, US)

Chapter 7. Immunophenotyping of Normal and Neoplastic Immature T-Cells
(Alexa J. Siddon, M.D., and Mina L. Xu, M.D., Department of Pathology & Laboratory Medicine, Yale University School of Medicine, New Haven, CT, US)

Chapter 8. Immunophenotyping of Neoplastic Mature T-Cells
Pawel Mroz, M.D., Ph.D.1*, Gabriela Gheorghe, M.D.2, David M. Dorfman, M.D., Ph.D.3, and
(Michael A. Linden, M.D., Ph.D, Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, MN, US)

Chapter 9. Flow Cytometric Immunophenotyping of NK-Cells
(Aaron R. Victor M.D., Ph.D., and Aharon G. Freud, M.D., Ph.D., Department of Pathology and Laboratory Medicine, Cedars-Sinai, Los Angeles, CA, US, and others)

Chapter 10. Introduction to Flow Cytometric Immunophenotyping of Myeloid Cells
(Xueyan Chen, M.D., Ph.D., and Sindhu Cherian, M.D., Department of Laboratory Medicine, University of Washington, Seattle, WA, US)

Chapter 11. Acute Myeloid Leukemia
(Katelyn Dannheim, M.D., Jason H. Kurzer, M.D., Ph.D., and Olga K. Weinberg, M.D., Department of Pathology, Beth Israel Deaconess Medical Center, Boston, MA, US, and others)

Chapter 12. Principles of Minimal Residual Disease Detection: Minimal Residual Disease Assessment in B-Lymphoblastic Leukemia
(Sunita Park, M.D., Department of Pathology, Children’s Healthcare of Atlanta, Atlanta, GA, US, and others)

Chapter 13. Minimal Residual Disease Assessment in Acute Myeloid Leukemia
(Xueyan Chen, M.D., Ph.D., and Sindhu Cherian, M.D., Department of Laboratory Medicine, University of Washington, Seattle, WA, US)

Chapter 14. Minimal Residual Disease Assessment in Plasma Cell Myeloma
(Irina Grigorieva, PhD, Flow Cytometry and Molecular Diagnostics Laboratories, Northside Hospital, Atlanta, GA, US)

Index

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