Chapter 6. A Review of Hydroxyapatite – Sustainable Product Development in Terms of Waste Valorization


Murugiah Krishani1, M. Tech, Hazwani Suhaimi1, PhD, and Nonni Soraya Sambudi2, PhD
1Faculty of Integrated Technologies, Universiti Brunei Darussalam Bandar Seri Begawan, Brunei Darussalam
2Department of Chemical Engineering, Universiti Teknologi Petronas, Seri Iskandar, Perak, Malaysia

Part of the book: What to Know about Hydroxyapatite


Million tons of shell waste are dumped in oceans and landfills and considered nuisance waste. Rather than disposing of the shells, recycling and reusing are excellent alternatives. In general, shells are rich in calcium carbonate, so sustainable development of calcium-based products may help reduce waste shell accumulation. Hydroxyapatite is a calcium phosphate-based mineral having similar composition and structure to natural human bone and teeth. Excellent biocompatibility, bioactivity, biodegradability, and non-toxicity; are some properties that enable hydroxyapatite to use as a superior biomaterial. The demand to obtain hydroxyapatite in a simple, efficient, scalable, and environmentally friendly way is increasing daily. Not only from shells but hydroxyapatite can also be synthesized from animal bones, fish scales, plants, and algae. This review discussed the highlights of hydroxyapatite and its natural sources, synthesis, and biomedical applications. Moreover, it also explained the importance of hydroxyapatite synthesized from biogenic waste materials. From this review, researchers may get an idea of the synthesis of hydroxyapatite from various sources in the development of biomedical applications and the conservation of food and agricultural waste management in terms of waste valorization.

Keywords: hydroxyapatite, waste valorization, biomaterial, waste management


Abdulrahman, I., Hamzat Ibiyeye Tijani, Bashir Abubakar Mohammed, Haruna Saidu,
Hindatu Yusuf, Mohammed Ndejiko Jibrin, and Sulaiman Mohammed. 2014. “From
Garbage to Biomaterials: An Overview on Egg Shell Based Hydroxyapatite.” Journal
of Materials 2014: 1-6.
Afriani, F., Y. Tiandho, J. Evi, A. Indriawati, and R. A. Rafsanjani. 2019. “Synthesis and
Characterization of Hydroxyapatite/Silica Composites Based on Cockle Shells Waste
and Tin Tailings.” IOP Conference Series: Earth and Environmental Science 353 (1).
Akpan, E. S., M. Dauda, L. S. Kuburi, D. O. Obada, and D. Dodoo-Arhin. 2020. “A
Comparative Study of the Mechanical Integrity of Natural Hydroxyapatite Scaffolds
Prepared from Two Biogenic Sources Using a Low Compaction Pressure Method.”
Results in Physics 17 (December 2019): 103051.
Akram, M., Rashid Ahmed, Imran Shakir, Wan Aini Wan Ibrahim, and Rafaqat Hussain.
2014. “Extracting Hydroxyapatite and Its Precursors from Natural Resources.”
Journal of Materials Science 49 (4): 1461-75.
Amini, A. R., Cato T. Laurencin, and Syam P. Nukavarapu. 2012. “Bone Tissue
Engineering: Recent Advances and Challenges.” Critical Reviews in Biomedical
Engineering 40 (5): 363-408.
Arokiasamy, P., Mohd Mustafa Al Bakri Abdullah, Shayfull Zamree Abd Rahim,
Salmabanu Luhar, Andrei Victor Sandu, Noorina Hidayu Jamil, and Marcin Nabiałek.
2022. “Synthesis Methods of Hydroxyapatite from Natural Sources: A Review.”
Ceramics International 48 (11): 14959-79.
Ashokan, A., Vivek Rajendran, T. S. Sampath Kumar, and Guhan Jayaraman. 2021.
“Eggshell Derived Hydroxyapatite Microspheres for Chromatographic Applications
by a Novel Dissolution – Precipitation Method.” Ceramics International 47 (13):
Bahrololoom, M. E., M. Javidi, S. Javadpour, and J. Ma. 2009. “Characterisation of Natural
Hydroxyapatite Extracted from Bovine Cortical Bone Ash.” J. Ceram. Process. Res
10 (2): 129-38.
Bakan, F., Oral Laçin, and Hanifi Sarac. 2013. “A Novel Low Temperature Sol-Gel
Synthesis Process for Thermally Stable Nano Crystalline Hydroxyapatite.” Powder
Technology 233: 295-302.
Barua, E., Ashish B. Deoghare, Payel Deb, and Sumit Das Lala. 2018. “Naturally Derived
Biomaterials for Development of Composite Bone Scaffold: A Review.” In IOP
Conference Series: Materials Science and Engineering. Vol. 377.
Biswal, T. 2020. “Biopolymers for Tissue Engineering Applications: A Review.” Materials
Today: Proceedings.
Brown, W. E., and L. C. Chow. 1976. “Chemical Properties of Bone Mineral.”
Annu Rev Mater Sci 6: 213-236.
Chocholata, P., Vlastimil Kulda, and Vaclav Babuska. 2019. “Fabrication of Scaffolds for
Bone-Tissue Regeneration.” Materials 12 (4).
Cox, S. C. 2015. “Synthesis Method of Hydroxyapatite.” Ceram, no. January 2014: 1-7.
Dehghan, M., Mohammad Khajeh Mehrizi, and Habib Nikukar. 2021. “Modeling and
Optimizing a Polycaprolactone/Gelatin/Polydimethylsiloxane Nanofiber Scaffold for
Tissue Engineering: Using Response Surface Methodology.” Journal of the Textile
Institute 112 (3): 482-93.
Deshmukh, K., M. Monsoor Shaik, Sutapa Roy Ramanan, and Meenal Kowshik. 2016.
“Self-Activated Fluorescent Hydroxyapatite Nanoparticles: A Promising Agent for
Bio-Imaging and Bio-Labeling Self-Activated Fluorescent Hydroxyapatite
Nanoparticles: A Promising Agent for Bio-Imaging and Bio-Labeling.” ACS
Biomaterials Science & Engineerin 2 (8): 1257-64.
Dorozhkin, S. V. 2017. “A History of Calcium Orthophosphates (CaPO4) and Their
Biomedical Applications.” Morphologie 101 (334): 143-53.
Ehrlich, H., Rajko Martinović, Danijela Joksimović, Iaroslav Petrenko, Stefano
Schiaparelli, Marcin Wysokowski, Dmitry Tsurkan, Allison L. Stelling, Armin
Springer, Michael Gelinsky & Aleksandar Joksimović. 2020. “Conchixes: Organic
Scaffolds Which Resemble the Size and Shapes of Mollusks Shells, Their Isolation
and Potential Multifunctional Applications.” Applied Physics A: Materials Science
and Processing 126 (7): 1-13.
Ferro, A. C., and Mafalda Guedes. 2019. “Mechanochemical Synthesis of Hydroxyapatite
Using Cuttlefish Bone and Chicken Eggshell as Calcium Precursors.” Materials
Science and Engineering C 97 (October 2018): 124-40.
Gomes, D. S., A. M. C. Santos, G. A. Neves, R. R. Menezes, Campina Grande, and
Campina Grande. 2019. “A Brief Review on Hydroxyapatite Production and Use in
Biomedicine.” Ceramica 65: 282-302.
Haider, A., Sajjad Haider, Sung Soo Han, and Inn Kyu Kang. 2017. “Recent Advances in
the Synthesis, Functionalization and Biomedical Applications of Hydroxyapatite: A
Review.” RSC Advances 7 (13): 7442-58.
Hembrick-Holloman, V., Temesgen Samuel, Zaheeruddin Mohammed, Shaik Jeelani, and
Vijaya K. Rangari. 2020. “Ecofriendly Production of Bioactive Tissue Engineering
Scaffolds Derived from Egg- And Sea-Shells.” Journal of Materials Research and
Technology 9 (6): 13729-39.
Jafari, S. 2015. “Application of Hydroxyapatite Nanoparticle in the Drug Delivery
Systems.” Journal of Molecular Pharmaceutics & Organic Process Research 03 (01):
Kalita, S. J., Abhilasha Bhardwaj, and Himesh A. Bhatt. 2007. “Nanocrystalline Calcium
Phosphate Ceramics in Biomedical Engineering.” Materials Science and Engineering
C 27 (3): 441-49.
Kara, A., Oylum C. Gunes, Aylin Z. Albayrak, Gokcen Bilici, Guven Erbil, and Hasan
Havitcioglu. 2020. “Fish Scale/Poly(3-Hydroxybutyrate-Co-3-Hydroxyvalerate)
Nanofibrous Composite Scaffolds for Bone Regeneration.” Journal of Biomaterials
Applications 34 (9): 1201-15.
Karacan, I., Besim Ben-Nissan, and Sutinee Sinutok. 2019. Marine-Based Calcium
Phosphates from Hard Coral and Calcified Algae for Biomedical Applications.
Springer Singapore.
Kumar, A., Saeid Kargozar, Francesco Baino, and Sung S Han. 2019. “Additive
Manufacturing Methods for Producing Hydroxyapatite and Hydroxyapatite-Based
Composite Scaffolds: A Review.” Frontiers in Materials 6.
Laonapakul, T. 2015. “Synthesis of Hydroxyapatite from Biogenic Wastes.” Kku
Engineering Journal 42 (3): 269-75.
Lim, S. S., Chye Jian Oon, Kit Wayne Chew, Jing Ying Yap, May Teng Lim, Derwin Su,
Lai Yee Lee, and Hwei San Loh. 2020. “Improved Physical Properties and in Vitro
Biocompatibility of Chitosan Composite Scaffolds Incorporated with a Green Filler
on Bone Cells.” Clean Technologies and Environmental Policy 22 (3): 701-12.
Lin, K., and J. Chang. 2015. Structure and Properties of Hydroxyapatite for Biomedical
Applications. Hydroxyapatite (Hap) for Biomedical Applications. Vol. 4214. Elsevier
Ma, Guoqing. 2019. “Three Common Preparation Methods of Hydroxyapatite.” IOP
Conference Series: Materials Science and Engineering 688 (3): 0-12.
Machado, T. R., I. S. Leite, N. M. Inada, M. S. Li, E. Cordoncillo, and E. Longo. 2019.
“Designing Biocompatible and Multicolor Fl Uorescent Hydroxyapatite Nanoparticles
for Cell-Imaging Applications.” Materials Today Chemistry 14.
Matsuda, Y., Tetsunari Nishikawa, Tomoharu Okamura, Kazuya Tominaga, Masahiro
Wato, Hajime Tabata, Makoto Umeda, Nobutaka Okusa, Koichi Imai, Akio Tanaka,
Isao Tamura 2017. “Comparative Study of Tissue Affinity, Chemical Characteristics
of Cultured and Natural Coral as a Bioabsorbable Scaffold.” Journal of Oral Tissue
Engineering 14 (3): 164-70.
Murugiah, K., M. I. Zakaria, H. Suhaimi, W. Caesarendra, and N. S. Sambudi. 2021.
“Synthesis and Characterisation of Hydroxyapatite (HAp) from Asiatic Hard Clam
(Meretrix Meretrix) and Blood Cockle Clam (Anadara Granosa) Using Wet
Precipitation Process.” In 2021 IEEE National Biomedical Engineering Conference
(NBEC), 1-6.
Oladele, I. O., O. G. Agbabiaka, O. G. Olasunkanmi, A. O. Balogun, and M. O. Popoola.
2018. “Non-Synthetic Sources for the Development of Hydroxyapatite.” Journal of
Applied Biotechnology & Bioengineering 5 (2): 88-95.
Pai, S., S. M Kini, R. Selvaraj, and A. Pugazhendhi. 2020. “A Review on the Synthesis of
Hydroxyapatite, Its Composites and Adsorptive Removal of Pollutants from
Wastewater.” J. Water Process Eng 38.
Pokhrel, S. 2018. “Hydroxyapatite: Preparation, Properties and Its Biomedical
Applications.” Advances in Chemical Engineering and Science 8: 225-40.
Pu’ad, N. A. S. Mohd, R. H. Abdul Haq, H. Mohd Noh, H. Z. Abdullah, M. I. Idris, and T.
C. Lee. 2019. “Synthesis Method of Hydroxyapatite: A Review.” Materials Today:
Proceedings 29: 233-239.
Rivera, E. M., Miguel Araiza, Witold Brostow, Victor M. Castaño, J. R. Díaz-Estrada, R.
Hernández, and J. Rogelio Rodríguez. 1999. “Synthesis of Hydroxyapatite from
Eggshells.” Materials Letters 41 (3): 128-34.
Sadat-Shojai, M., Mohammad Taghi Khorasani, Ehsan Dinpanah-Khoshdargi, and Ahmad
Jamshidi. 2013. “Synthesis Methods for Nanosized Hydroxyapatite with Diverse
Structures.” Acta Biomaterialia 9 (8): 7591-7621.
Saleem, M., Sidra Rasheed, and Chen Yougen. 2020. “Silk Fibroin/Hydroxyapatite
Scaffold: A Highly Compatible Material for Bone Regeneration.” Science and
Technology of Advanced Materials 21 (1): 242-66.
Sari, M., Puspa Hening, Chotimah, Ika Dewi Ana, and Yusril Yusuf. 2021. “Porous
Structure of Bioceramics Carbonated Hydroxyapatite-Based Honeycomb Scaffold for
Bone Tissue Engineering.” Materials Today Communications 26 (October 2020):
Shackelford, J. F. 1999. “Bioceramics – an Historical Perspective.” Materials Science
Forum 293: 1-4.
Shi, P., Meng Liu, Fengjiao Fan, Cuiping Yu, Weihong Lu, and Ming Du. 2018.
“Characterization of Natural Hydroxyapatite Originated from Fish Bone and Its
Biocompatibility with Osteoblasts.” Materials Science and Engineering C 90 (March):
Suresh Kumar, C., K. Dhanaraj, R. M. Vimalathithan, P. Ilaiyaraja, and G. Suresh. 2020.
“Hydroxyapatite for Bone Related Applications Derived from Sea Shell Waste by
Simpleprecipitation Method.” Journal of Asian Ceramic Societies 8 (2): 416-29.
Szcześ, A., Lucyna Hołysz, and Emil Chibowski. 2017. “Synthesis of Hydroxyapatite for
Biomedical Applications.” Advances in Colloid and Interface Science 249 (April):
Urist, M. R. 1965. “Bone: Formation by Autoinduction.” Science (New York, N.Y.) 150
(3698): 893-99.
Wang, Z., L. Han, T. Sun, W. Wang, X. Li, and B. Wu. 2021. “Construction of Tissue Engineered
Bone with Differentiated Osteoblasts from Adipose-Derived Stem Cell
and Coral Scaffolds at an Ectopic Site.” British Journal of Oral and Maxillofacial
Surgery 59 (1): 46-51.
Wijedasa, N. P., Sarah M. Broas, Rachel E. Daso, and Ipsita A. Banerjee. 2020. “Varying
Fish Scale Derived Hydroxyapatite Bound Hybrid Peptide Nanofiber Scaffolds for
Potential Applications in Periodontal Tissue Regeneration.” Materials Science and
Engineering C 109 (August 2019): 110540.
Zaman, S. U., Muhammad Khaliq, U Zaman, and Nawshad Muhammad. 2020. “Overview
of Hydroxyapatite; Composition, Structure, Synthesis Methods and Its Biomedical
Uses.” Biomedical Letters 6 (1): 84-99.


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