Cellular Interactions of Probiotic Bacteria with Intestinal and Immune Cells

Sarah Moore and Kasipathy Kailasapathy
Western Sydney University, Australia

Series: Bacteriology Research Developments
BISAC: SCI006000



Volume 10

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Volume 2

Volume 3

Special issue: Resilience in breaking the cycle of children’s environmental health disparities
Edited by I Leslie Rubin, Robert J Geller, Abby Mutic, Benjamin A Gitterman, Nathan Mutic, Wayne Garfinkel, Claire D Coles, Kurt Martinuzzi, and Joav Merrick


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This book contains novel research and laboratory techniques to study the immune cell molecular behaviour and responses to soluble factors produced by probiotic bacteria and intestinal/probiotic co-cultures that will contribute to advanced scientific knowledge and the commercial development of probiotics. Such understanding will allow scientists to identify new probiotic strains with enhanced immunomodulatory effects, ascertain minimal probiotic dose requirements, distinguish the type of immunological response to probiotics and identify potential probiotic soluble factors that induce immunological reactions.

This book includes recent techniques involved in the use of adaptive immune cells with intracellular expressed fluorescent proteins acting as bio-indicators of responses to soluble factors produced by probiotic strains and intestinal/probiotic co-cultures. Research techniques on the immune cell response to the microencapsulated probiotics is described as a downstream indicator to determine changes in the behaviour of the confined probiotics. Changes in probiotic bacterial-activities from the microencapsulated environment have rarely been reported and are evaluated in this book using the transfected porcine progenitor immune cells as a downstream indicator. To date, no studies have reported any form of immunological responses (innate or adaptive) to microencapsulated probiotic bacteria. These results are compared to the responses of immune cells treated with non-microencapsulated probiotic bacteria.

This book also contains advanced fluorescence microscopy techniques to obtain real-time information from viable cells. Fluorescent proteins have been widely used as bio-indicators of cellular responses. The porcine progenitor immune cells can be transfected with the fluorescent proteins, phMGFP and pCIneo-DsRed2. Changes in fluorescence and diffusive properties of these fluorescent proteins in response to the probiotic bacterial-produced soluble factors can be imaged by LSCM and FCS, and analysed using the RICS program. These changes can be compared to the immune cellular responses to pathogen-produced soluble factors (Streptococcus pyogenes). The intracellular responses of the immune cells to the probiotics and pathogen are compared to determine if the probiotic strains exhibited similar effects on immune cellular responses.

This book is ideally suited for researchers in food and pharmaceutical industries as well as universities; postgraduate students in microbiology and immunology will also find this book to be useful. This book is suitable as a classroom textbook for advanced microbiology, bacteriology and immunology, and it is highly recommended for university and research institution libraries around the world. (Imprint: Nova Biomedical)


Chapter 1. Introduction

Chapter 2. Growth of Probiotic Bacteria in Mammalian Cell Culture Conditions and Confocal Microscopy - Analysis of Microencapsulated Probiotic Bacteria

Chapter 3. Development of a Fluorescent Immune Cell Model

Chapter 4. Free and Microencapsulated Probiotic Bacteria- Effects on Immune Cell Proliferation

Chapter 5. Intestinal Cell Co-cultures with Free and Microencapsulated Probiotic Bacteria - Effects on Immune Cell Proliferation

Chapter 6. Free and Microencapsulated Probiotic Bacteria- Effects on Cytokine and CD Marker Gene Expression by Immune Cells

Chapter 7. Immune Intracellular Fluorescent Protein Diffusion Responses to Free and Microencapsulated Probiotic Bacteria

Chapter 8. General Discussion and Future Directions



About the Authors


“Professor Kailasapathy is one of the foremost and outstanding experts in the microencapsulation of probiotic bacteria and their incorporation into foods and feeds. This book presents valuable information on the effect of microencapsulation on the functionality of probiotic bacteria on immune cells of the gastro-intestinal system. In particular it sheds light on the mechanisms by which probiotics exert their beneficial effects on the porcine immune system. It is therefore recommended reading for students and scientists involved in improving animal welfare through probiotics.” - Dr Claude P. Champagne, Agriculture and Agri-food Canada, Saint-Hyacinthe, QC

"Professor Kailasapathy is a world leading expert researcher in probiotics and microencapsulation and this contribution testify his knowledge in this field. The entire book is satisfactorily presented in text, tables and figures. This will be of immense use to researchers and scholars working in the area of probiotics and health. It will also give some insight to the industry who wish to exploits probiotics for human health." - J. B. Prajapati, Professor of Dairy Microbiology, Principal & Dean, Faculty of Dairy Science, Anand Agricultural University, India

Afrin, R., Zohora, U. S., Uehara, H., Watanabe-Nakayama, T. & Ikai, A. (2009) Atomic force microscopy for cellular level manipulation: imaging intracellular structures and DNA delivery through a membrane hole. Journal Of Molecular Recognition,

Ahmad, N., Drew, W. L. & Plorde, J. J. (2010) Sherris medical microbiology, New York, USA, The McGrath-Hill Companies.
Allan-Wojtas, P., Truelstrup Hansen, L., Paulson, A. T. & Baird, G. S. (2008) Microstructural studies of probiotic bacteria-loaded alginate microcapsules using standard electron microscopy techniques and anhydrous fixation. LWT,

Almond, B. D. (2003) Monster Green® Protein: a brighter, longer-expressing green fluorescent protein. Promega Notes,

Altan, N., Chen, Y., Schindler, M. & Simon, S. M. (1998) Defective acidification in human breast tumor cells and implications for chemotherapy. Journal of Experimental Medicine,

Amrouche, T., Boutin, Y., Prioult, G. & Fliss, I. (2006) Effects of bifidobacterial cytoplasm,cell wall and exopolysaccharide on mouse lymphocyte proliferation and cytokine production. International Dairy Journal,

Anal, A. K. & Singh, H. (2007) Recent advances in microencapsulation of probiotics for industrial applications and targeted delivery. Trends in Food Science & Technology,

Anderson, J. W. & Gilliland, S. E. (1999) Effect of fermented milk (Yogurt) containing Lactobacillus acidophilus L1 on serum cholesterol in hypercholesterolemic humans. Journal of the American College of Nutrition,

Annan, N. T., Borza, A., Moreau, D. L., Allan-Wojtas, P. M. & Truelstrup Handsen, L. (2007) Effect of process variables on particle size and viability of Bifidobacterium lactis Bb-12 in genipin-gelatin microspheres. Journal of Microencapsulation,

Arunachalam, K., Gill, H.S. & Chandra, R.K. (2000) Enhancement of natural immune function by dietary consumption of Bifidobacterium lactis (HN019). European Journal of Clinical Nutrition,

Baird, G. S., Zacharias, D. A. & Tsien, R. Y. (2000) Biochemistry, mutagenesis, and oligomerization of DsRed, a red fluorescent protein from coral. PNAS,

Bell, P., Vandenberghe, L. H., Wu, D., Johnston, J., Limberis, M. & Wilson, J. M. (2007) A comparative analysis of novel fluorescent proteins as reporters for gene transfer studies. Journal of Histochemistry and Cytochemistry,

Benyacoub, J., Czarnecki-Maulden, G. L., Cavadini, C., Sauthier, T., Anderson, R. E., Schiffrin, E. J. & Von Der Weid, T. (2003) Supplementation of food with Enterococcus faecium (SF68) stimulates immune functions in young dogs. Journal of Nutritional Immunology,

Berg, R. D. (1998) Probiotics, prebiotics or 'conbiotics'? Trends In Microbiology,

Berney, M., Hammes, F., Bosshard, F., Weilenmann, H. & Egli, T. (2007) Assessment and interpretation of bacterial viability by using the Live/Dead BacLight kit in combination with flow cytometry. Applied and Environmental Microbiology,

Betz, N. & Farfan, A. (2003) Transfecting a human neuroblastoma cell line with Monster Green® Fluorescent Protein. Neuroscience,

Bhathena, J., Martoni, C., Kulamarva, A., Urbanska, A. M., Malhotra, M. & Prakash, S. (2009) Orally delivered microencapsulated live probiotic formulation lowers serum lipids in hypercholesterolemic hamsters. Journal of Medicinal Food,

Biondvax Pharmaceuticals LTD (2007) http://www.biondvax.com/ [Accessed 5.2.07]
Bitplane. (2011) http://www.bitplane.com/go/products/imaris [Accessed 28.6.11]Bjorksten, B. (2006) The gut microbiota: a complex ecosystem. Clinical and Experimental Allergy,

Blandino, A., Macias, M. & Cantero, D. (1999) Formation of calcium alginate gel capsules: Influence of sodium alginate and CaCl2 concentration on gelation kinetics. Journal of Bioscience and Bioengineering,

Blum, S., Haller, D., Pfeifer, A. & Schiffrin, E. J. (2002) Probiotics and immune response. Clinical Reviews in Allergy and Immunology,

Blum, S. & Schiffrin, E. J. (2003) Intestinal microflora and homeostasis of the mucosal immune response: implications for probiotic bacteria? Current Issues in Intestinal Microbiology,

Boirivant, M. & Strober, W. (2007) The mechanism of action of probiotics. Current Opinion in Gastroenterology,

Bokman, S. H. & Ward, W. W. (1981) Renaturation of Aeuqorea green-fluorescent protein. Biochemical and Biophysical Research Communications,

Boudeau, J., Glasser, A-L., Julien, S., Colombel, J-F. & Darfeuille-Michaud, A. (2003). Inhibitory effect of probiotic Escherichia coli strain Nissle 1917 on adhesion to and invasion of intestinal epithelial cells by adherent-invasive E. coli strains isolated from patients with Crohn’s Disease. Alimentary Pharmacology & Therapeuticsm

Boulos, L., Prevost, M., Barbeau, B., Coallier, J. & Desjardins, R. (1999) LIVE/DEAD® BacLight™: application of a new rapid staining method for direct enumeration of viable and total bacteria in drinking water. Journal of Microbiological Methods,

Bowman, S. (2008) Developing a model to characterise progenitor cell specific gene therapy. College of Health and Sciences. Hawkesbury, Australia, University of Western Sydney.
Braat, H., Van Der Brande, J., Van Tol, E., Hommes, D., Peppelenbosch, M. & Van Deventer, S. (2004) Lactobacillus rhamnosus induces peripheral hyporesponsiveness in stimulated CD4+ T cells via modulation of dendritic cell function. American Journal of Clinical Nutrition,

Britti, M. S., Roselli, M., Finamore, A., Merendino, N. & Mengheri, E. (2006) Regulation of immune response at intestinal and peripheral sites by probiotics. Biologica - Section Cellular and Molecular Biology,

Brown, C. M., Dalal, R. B., Herbert, B., Digman, M. A., Horwitz, A. R. & Gratton, E. (2008) Raster image correlation spectroscopy (RICS) for measuring fast protein dynamics and concentrations with a commercial laser scanning confocal microscope. Journal of Microscopy,

Bujalance, C., Moreno, E., Jimenez-Valera, M. & Ruiz-Bravo, A. (2007) A probiotic strain of Lactobacillus plantarum stimulated lymphocyte responses in immunologically intact and immunocompromised mice. International Journal of Food Microbiology,

Burdin, N., Van Kooten, C., Galibert, L., Abrarns, J. S., Wijdenes, J., Banchereau, J. & Rowset, F. (1995) Endogenous IL-6 and IL-10 contribute to the differentiation of CD40- activated human B lymphocytes. Journal of Immunology,

Burke, J. P., Cunningham, M. F., Watson, R. W. G., Docherty, N. G., Coffey, J. C. & O'connell, P. R. (2010) Bacterial lipopolysaccharide promotes profibrotic activation of intestinal fibroblasts. British Journal of Surgery,

Buyukgungor, H. (1992) Stability of Lactobacillus bulgaricus immobilized in K-carrageenan gels. Journal of Chemical Technology and Biotechnology,

Campbell, T. N. & Choy, F. Y. M. (2001) The effect of pH on green fluorescent protein: a brief review. Molecular Biology Today,

Canny, G., Swidsinski, A. & Mccormick, B. A. (2006) Interactions of intestinal epithelial cells with bacteria and immune cells: methods to characterize microflora and functional consequences. Methods in Molecular Biology,

Cencic, A. (2010) The prosperous future in gut research - functional cell models of the GIT. International Scientific Conference Probiotics and Prebiotics. Kosice, Slovakia.
Cerutti, A. (2008) The regulation of IgA class switching. Nature Reviews Immunology,

Chalfie, M., Tu, Y., Euskirchen, G., Ward, W. W. & Prasher, D. C. (1994) Green fluorescent protein as a marker for gene expression. Science,

Chandramouli, V., Kailasapathy, K., Peiris, P. & Jones, M. (2004) An improved method of microencapsulation and its evaluation to protect Lactobacillus spp. in simulated gastric conditions. Journal of Microbiological Methods,

Chavarri, M., Maranon, I., Ares, R., Ibanez, F. C., Marzo, F. & M.D.C., V. (2010) Microencapsulation of a probiotic and prebiotic in alginate-chitosan capsules improves survival in simulated gastrointestinal conditions. International Journal of Food Microbiology,

Chen, A., Zheng, G. & Tykocinski, M.L. (2000) Hierarchical costimulator thresholds for distinct immune responses: applications of a novel two-step Fc fusion protein transfer method. The Journal of Immunology,

Chen, Y., Muller, J. D., So, P. T. C. & Gratton, E. (1999) The photon counting histogram in fluorescence fluctuation spectroscopy. Biophysics Journal,

Christensen, H. R., Frøkiær, H. & Pestka, J. J. (2002) Lactobacilli differentially modulate expression of cytokines and maturation surface markers in murine dendritic cells. The Journal of Immunology,

Ciorba, M. A., Riehl, T. E., Rao, M. S., Moon, C., Ee, X., Nava, G. M., Walker, M. R., Marinshaw, J. M., Stappenbeck, T. S. & Stenson, W. F. (2011) Lactobacillus probiotic protects intestinal epithelium from radiation injury in a TLR-2/cyclo-oxygenase-2-dependent manner. Gut, Epub.

Clancy, R. L., Gleeson, M., Cox, A., Callister, R., Dorrington, M., D'este, C., Pang, G., Pyne, D., Fricker, P. & Henriksson, A. (2006) Reversal in fatigued athletes of a defect in interferon gamma-secretion after administration of Lactobacillus acidophilus. Br J Sports Med,

Claxton, N. S., Fellers, T. J. & Davidson, M. W. (2006) Laser scanning confocal microscopy. http:www.olympusfluoview.com/theory/LSCMIntro.pdf.
Collins, J. K., Thornton, G. & O'sullivan, G. O. (1998) Selection of probiotic strains for human applications. International Dairy Journal,

Coombes, J. L. & Powrie, F. (2008) Dendritic cells in intestinal immune regulation. Nature Reviews Immunology,

Corcoran, B. M., Stanton, C., Fitzgerald, G. & Ross, R. P. (2008) Life under stress: the probiotic stress response and how it may be manipulated. Current Pharmaceutical Design,

Cormack, B. P., Valdivia, R. H. & Falkow, S. (1996) FACS-optimized mutants of the green fluorescent protein (GFP). Gene,

Corthesy, B., Gaskins, H. R. & Mercenier, A. (2007) Cross-talk between probiotic bacteria and the host immune system. The Journal of Nutrition,

Costes, S. V., Daelemans, D., Cho, E. H., Dobbin, Z., Pavlakis, G. & Lockett, S. (2004) Automatic and quantitative measurement of protein-protein colocalization in live cells. Biophysical Journal,

Cotran, R., Kumar, V. & Collins, T. (1999) Robbins pathological basis of disease, Philadelphia, PA, WB Saunders.
Cross, M. L., Ganner, A., Teilab, D. & Fray, L. M. (2004) Patterns of cytokine induction by gram-positive and gram-negative probiotic bacteria. FEMS Immunology and Medical Microbiology,

Curran, H. R., Rogers, L. A. & Whittier, E. O. (1932) The distinguishing characteristics of Lactobacillus acidophilus. Journal of Bacteriology,

De Angelis, M. & Gobbetti, M. (2004) Environmental stress responses in Lactobacillus: a review. Proteomics,

De Giorgi, F., Brini, M., Bastianutto, C., Marsault, R., Montero, M., Pizzo, P., Rossi, R. & Rizzuto, R. (1996) Targeting aequorin and green fluorescent protein to intracellular organelles. Gene,

De Moreno De Leblanc, A., Chaves, S., Carmuega, E., Weill, R., Antoine, J. & Perdigon, G. (2008) Effect of long-term continuous consumption of fermented milk containing probiotic bacteria on mucosal immunity and the activity of peritoneal macrophages. Immunobiology,

Delcenserie, V., Martel, D., Lamoureux, M., Amiot, J., Boutin, Y. & Roy, D. (2008) Immunomodulatory effects of probiotics in the intestinal tract. Current Issues in Molecular Biology,

Di Giacinto, D., Marinaro, M., Sanchez, M., Strober, W. & Boirivant, M. (2005) Probiotics ameliorate recurrent Th1-mediated murine colitis by inducing IL-10 and IL-10-dependent TGF-B-bearing regulatory cells. Journal of Immunology,

Digman, M. A., Brown, C. M., Sengupta, P., Wiseman, P. W., Horwitz, A. R. & Gratton, E. (2005a) Measuring fast dynamics in solutions and cells with a laser scanning microscope. Biophysical Journal,

Digman, M. A. & Gratton, E. (2009) Analysis of diffusion and binding in cells using the RICS approach. Microscopy Research and Technique,

Digman, M. A. & Gratton, E. (2011) Lessons in fluctuation correlation spectrosopy. Annu. Rev. Phys. Chem.,

Digman, M. A., Sengupta, P., Wiseman, P. W., Brown, C. M., Horwitz, A. R. & Gratton, E. (2005b) Fluctuation correlation spectroscopy with a laser-scanning microscope: exploiting the hidden time structure. Biophysical Journal,

Ding, W. K. & Shah, N. P. (2008) Survival of free and microencapsulated probiotic bacteria in orange and apple juices. International Food Research Journal,

Dogi, C. A., Galdeano, C. M. & Perdigon, G. (2008) Gut immune stimulation by non pathogenic Gram(+) and Gram(-) bacteria. Comparison with a probiotic strain. Cytokine,

Dogi, C. A. & Perdigon, G. (2006) Importance of the host specificity in the selection of probiotic bacteria. Journal of Dairy Research,

Dogi, C. A., Weill, F. & Perdigon, G. (2010) Immune response of non-pathogenic gram(+) and gram(-) bacteria in inductive sites of the intestinal mucosa. Study of the pathway of signaling involved. Immunobiology,

Dong, H., Rowland, I., Tuohy, K. M., Thomas, L. V. & Yaqoob, P. (2010) Selective effects of Lactobacillus casei Shirota on T cell activation, natural killer cell activity and cytokine production. Clinical and Experimental Immunology,

Dong, H., Rowland, I. & Yaqoob, P. (2011) Comparative effects of six probiotic strains on immune function in vitro. British Journal of Nutrition,

First view:1-12.
Dooley, C. T., Dore, T. M., Hanson, G. T., Jackson, W. C., Remington, S. J. & Tsien, R. Y. (2004) Imaging dynamic redox changes in mammalian cells with green fluorescent protein indicators. The Journal of Biological Chemistry,

279(21):22284 -93.
Dozois, C. M., Oswald, E., Gautier, N., Serthelon, J.-P., Fairbrother, J. M. & Oswald, I. P. (1997) A reverse transcription-polymerase chain reaction method to analyze porcine cytokine gene expression. Veterinary Immunology and Immunopathology,

Dunne, C., O'mahony, L., Murphy, L., Thornton, G., Morrissey, D., O'halloran, S., Feeney, M., Flynn, S., Fitzgerald, G., Daly, C., Kiely, B., O'sullivan, G. C., Shanahan, F. & Collins, J. K. (2001) In vitro selection criteria for probiotic bacteria of human origin: correlation with in vivo findings. American Journal of Clinical Nutrition,

Duvigneau, J. C., Hartl, R. T., Groiss, S. & Gemeiner, M. (2005) Quantitative simultaneous multiplex real-time PCR for the detection of porcine cytokines. Journal of Immunological Methods,

Dwyer, J. M. & Johnson, C. (1981) The Use Of Concanavalin A To Study The Immunoregulation Of Human T Cells. Clinical And Experimental Immunology,

Eckburg, P. B., Bik, E. M., Bernstein, C. N., Purdom, E., Dethlefsen, L., Sargent, M., Gill, S. R., Nelson, K. E. & Relman, D. A. (2005) Diversity of the human intestinal microbial flora. Science,

Elgert, K. D. (1996) Immunology. Understanding the immune system, New York, Wiley-Liss Inc.
Evrard, B., Coudeyras, S., Dosgilbert, A., Charbonnel, N., Alame, J., Tridon, A. & Forestier, C. (2011) Dose-dependent immunomodulation of human dendritic cells by the probiotic Lactobacillus rhamnosus Lcr35. PLoS ONE,

Ewaschuk, J. B., Diaz, H., Meddings, L., Diederichs, B., Dmytrash, A., Backer, J., Looijer-Van Langen, M. & Madsen, K. L. (2008) Secreted bioactive factors from Bifidobacterium infantis enhance epithelial cell barrier function. American Journal of Physiology. Gastrointestinal and Liver Physiology,

Ewing, W. N. & Cole, D. J. A. (1994) The Living Gut: an introduction to micro-organisms in nutrition, Ireland, Context Publication.
FAO/WHO (2001) Joint FAO/WHO expert consultation on evaluation of health and nutritional properties of probiotics in food including powder milk with live lactic acid bacteria. FAO/WHO (Food and Agriculture Organization/World Health Organization). Cordoba, Argentina: WHO: 2001.
Ferguson, L. R. & Denny, W. A. (2007) Genotoxicity of non-covalent interactions: DNA intercalators. Mutation Research,

Fernandez´, M. & Zu´ Niga˜, M. (2006) Amino acid catabolic pathways of lactic acid bacteria. Critical Reviews in Microbiology,

Foligne, B., Zoumpopoulou, G., Dewulf, J., Younes, A. B., Chareyre, F., Sirard, J.-C., Pot, B. & Grangette, C. (2007) A key role of dendritic cells in probiotic functionality. PLoS ONE,

Fujii, A. & Cook, E. S. (1973) Probiotics. Antistaphylococcal and antifibrinolytic activities of omega-guanidino acids and omega-guanidinoacyl-L-histidines. Journal of Medicinal Chemistry,

Fuller, R. (1989) Probiotics in man and animals. Journal of Applied Bacteriology,

Fuller, R. (1992) Problems and prospects. IN FULLER, R. (Ed.) Probiotics, the scientific basis. London, Chapman & Hall.
Ghadimi, D., Folster-Holst, R., De Vrese, M., Winkler, P., Heller, K. J. & Schrezenmeir, J. (2008) Effects of probiotic bacteria and their genomic DNA on Th1/Th2-cytokine production by peripheral blood mononuclear cells (PMBCs) of healthy and allergic subjects. Immunobiology,

Gill, H. S., Rutherford, K. J., Prasad, J. & Gopal, P. K. (2000) Enhancement of natural and acquired immunity by Lactobacillus rhamnosus (HN001), Lactobacillus acidophilus (HN017) and Bifidobacterium lactis (HN019). British Journal of Nutrition,

Gill, H. S., Rutherfurd, K. J., Cross, M. L. & Gopal, P. K. (2001) Enhancement of immunity in the elderly by dietary supplementation with the probiotic Bifidobacterium lactis HN019. Am J Clin Nutr,

Gillingham, L. G. & Lescheid, D. W. (2009) Probiotics and mucosal immunity: strain-specific effects on Th1/Th2 cell modulation. International Journal of Naturopathic Medicine,

Gomes, A. M. P. & Malcata, F. X. (1999) Bifidobacterium spp. and Lactobacillus acidophilus: biochemical, technological and therapeutical properties relevant for use as probiotics. Trends in Food Science & Technology,

Gonzalez-Gonzalez, E., Ra, H., Hickerson, R. P., Wang, Q., Piyawattanametha, W., Mandella, M. J., Kino, G. S., Lea, Ke, D., Avilion, A. A., Solgaard, O., Doyle, T. C., Contag, C. H. & Kaspar, R. L. (2009) siRNA silencing of keratinocyte-specific GFP expression in a transgenic mouse skin model. Gene Therapy,

Grollman, A. P. (1967) Inhibitors of protein synthesis. II. Mode of action of anisomycin. The Journal of Biological Chemistry,

Guarner, F. (2006) Enteric flora in health and disease. Digestion,

73(Suppl 1):5-12.
Guarner, F. & Malagelada, J.-R. (2003) Gut flora in health and disease. Lancet,

Guarner, F. & Schaafsma, G. J. (1998) Probiotics. International Journal of Food Microbiology,

Guerin, D., Vuillemard, J.-C. & Subirade, M. (2003) Protection of bifidobacteria encapsulated in polysaccharide-protein gel beads against gastric juice and bile. Journal of Food Protection,

Hacini-Rachinel, F., Nancey, S., Boschetti, G., Sardi, F., Doucet-Ladeveze, R., Durand, P.-Y., Flourie, B. & Kaiserlian, D. (2009) CD4+ T cells and Lactobacillus casei control relapsing colitis mediated by CD8+ T cells. The Journal of Immunology,

Haller, D., Bode, C., Hammes, W. P., Pfeifer, A. M., Schriffrin, E. J. & Blum, S. (2000) Non-pathogenic bacteria elicit a differential cytokine response by intestinal epithelial cell/leucocyte co-cultures. Gut,

Haller, D., Serrant, P., Peruisseau, G., Bode, C., Hammes, W. P., Schiffrin, E. & Blum, S. (2002) IL-10 producing CD14low monocytes inhibit lymphocyte-dependent activation of intestinal epithelial cells by commensal bacteria. Microbiology and Immunology,

Hamilton-Miller, J. M. T., Gibson, G. R. & Bruck, G. R. (2003) Some insights into the derivation and early uses of the word 'probiotic'. British Journal of Nutrition,

Haugland, R. P. (2005) The Handbook: a guide to fluorescent probes and labeling technologies, Chicago, USA, Invitrogen Molecular Probes.
Havenaar, R., Brink, B. T. & Huis In't Veld, J. H. J. (1992) Selection of strains for probiotic use. IN FULLER, R. (Ed.) Probiotics: The scientific basis. London, UK, Chapman & Hall.
He, F., Morita, H. & Ouwehand, A. C. (2006) Bifidobacteria and Lactobacilli exhibited different mitogenic activity on murine splenocytes. International Journal of Probiotics and Prebiotics,

Herick, R. & Levkut, M. (2002) Lactic acid bacteria, probiotics and immune system. Vet. Med. - Czech,

Holt, J. G., Krieg, N. R., Sneath, P. H. A., Staley, J. T. & Williams, S. T. (1994) Bergey's manual of determinative bacteriology, Maryland, USA, Williams & Wilkins.
Holzapfel, W. H., Haberer, P., Snel, J., Schillinger, U. & Huis In't Veld, J. H. J. (1998) Overview of gut flora and probiotics. International Journal of Food Microbiology,

Hong, S., Wang, Y.-N., Yamaguchi, H., Sreenivasappa, H., Chou, C.-K., Tsou, P.-H., Hung, M.-C. & Kameoka, J. (2010) Measurement of protein 53 diffusion coefficient in live HeLa cells using raster image correlation spectroscopy (RICS). Journal of Biomaterials and Nanobiotechnology,

Hua, M.-C., Lin, T.-Y., Lai, M.-W., Kong, M.-S., Chang, H.-J. & Chen, C.-C. (2010) Probiotic Bio-Three induces Th1 and anti-inflammatory effects in PBMC and dendritic cells. World Journal of Gastroenterology,

Ibnou-Zekri, N., Blum, S., Schiffrin, E. J. & Von Der Weid, T. (2003) Divergent patterns of colonization and immune response elicited from two intestinal Lactobacillus strains that display similar properties in vitro. Infection and Immunity,

Iordanov, M. S., Pribnow, D., Magun, J. L., Thank-Hoai Dindh, M., Pearson, J. A., Chen, S. L.-Y. & Magun, B. E. (1997) Ribotoxic stress response: activation of the stress-activated protein kinase JNK1 by inhibitors of the peptidyl transferase reaction and by sequence-specific RNA damage to the a-sarcin/ricin loop in the 28S rRNA. Molecular and Cellular Biology,

Isolauri, E., Sutas, Y., Kankaanpaa, P., Arvilommi, H. & Salminen, S. (2001) Probiotics: effects on immunity. Am J Clin Nutr,

Iwasaki, A. & Kelsall, B. L. (1999) Freshly isolated Peyer's patch, but not spleen, dendritic cells produce interleukin 10 and induce the differentiation of T helper type 2 cells. The Journal of Experimental Medicine,

Iwasaki, A. & Kelsall, B. L. (2000) Localization of distinct Peyer's patch dentritic cell subsets and their recruitment by chemokines macrophages inflammatory protein (MIP-3) alpha, MIP-3 beta, and secondary lymphoid organ chemokine. J. Exp. Med.,

Iyer, C., Phillips, M. & Kailasapathy, K. (2005) Release studies of Lactobacillus casei strain Shirota from chitosan-coated alginate-starch microcapsules in ex vivo porcine gastrointestinal contents. Letters in Applied Microbiology,

Johnson, I. (1998) Fluorescent probes for living cells. Histochemistry,

Kaeffer, B. (1994) Immortal porcine lymphoblastoid cell lines: interest for veterinary and medical research. Vet Res,

Kaeffer, B., Bottreau, E., Thanh, L. P., Olivier, M. & Salmon, H. (1990) Histocompatible miniature boar model: selection of transformed cell lines of B and T lineages producing retrovirus. International Journal of Cancer,

Kaeffer, B., Bottreau, E., Velge, P. & Pardon, P. (1993) Epithelioid and fibroblastic cell lines derived from the ileum of an adult histocompatible miniature boar (d/d haplotype) and immortalized by SV40 plasmid. European Journal of Cell Biology,

Kaeffer, B., Uriel, I. G. & Bottreau, E. (1994) Effects of hypothermia on the survival and cryopreservation of minipig ileal cells and Chinese hamster ovary cells. Cell Biology International,

Kailasapathy, K. (2002) Microencapsulation of probiotic bacteria: technology and potential applications. Current Issues in Intestinal Microbiology,

Kailasapathy, K. (2003) Protecting probiotics by microencapsulation. Microbiology Australia,

Kailasapathy, K. (2006) Survival of free and encapsulated probiotic bacteria and their effect on the sensory properties of yoghurt. Lebensmittel-Wissenschaft + Technologie,

Kailasapathy, K. (2008) Formulation, administration, and delivery of probiotics. In Versalovic, J. & Wilson, M. (Eds.) Therapeutic microbiology: probiotics and related strategies. Washington, DC, USA, ASM Press.
Kailasapathy, K. & Chin, J. (2000) Survival and therapeutic potential of probiotic organisms with reference to Lactobacillus acidophilus and Bifidobacterium spp. Immunology and Cell Biology,

Kailasapathy, K. & Godward, G. (2001) Micro-encapsulation of probiotic bacteria with alginate-starch and evaluation of their survival in dairy products. International Congress of Probiotics Medicine. Los Angeles, California.
Kailasapathy, K., Harmstorf, I. & Phillips, M. (2008) Survivial of Lactobacillus acidophilus and Bifidobacterium animalis ssp. lactis in stirred fruit yogurts. LWT,

Kailasapathy, K., Supriadi, D. & Hourigan, J. A. (1996) Effect of partially replacing skim milk powder with whey protein concentrate on buffering capacity of yoghurt. The Australian Journal of Dairy Technology,

Kandler, O. (1983) Carbohydrate metabolism in lactic acid bacteria. An tonie van Leeuwenhoek,

Kanzato, H., Fujiwara, S., Ise, W., Kaminogawa, S., Sato, R. & Hachimura, S. (2008) Lactobacillus acidophilus strain L-92 induces apoptosis of antigen-stimulated T cells by modulating dendritic cell function. Immunobiology,

Katayama, H., Yamamoto, A., Mizushima, N., Yoshimori, T. & Miyawaki, A. (2008) GFP-like proteins stably accumulate in lysosomes. Cell Structure and Function,

Kebary, K. M. K., Hussein, S. A. & Badawi, R. M. (1998) Improving viability of bifidobacteria and their effect on frozen milk. Egyptian Journal of Dairy Science,

Kidd, P. (2003) Th1/Th2 balance: the hypothesis, its limitations and implications for health and disease. Alternative Medicine Review,

Kim, S.-J., Cho, S.Y., Kim, S.H., Song, O-J., Shin, I-S., Cha, D.S. & Park, H.J. (2008) Effect of microencapsulation on viability and other characteristics in Lactobacillus acidophilus ATCC 43121. LWT,

Kindt, T. J., Goldsby, R. A. & Osborne, B. A. (2007) Kuby Immunology, New York, USA, W.H. Freeman and Company.
Kirjavainen, P. V., El-Nezami, H. S., Salminen, S. J., Akohas, J. T. & Wright, P. F. A. (1999) The effect of orally administered viable probiotic and dairy lactobacilli on mouse lymphocyte proliferation. FEMS Immunology and Microbiology,

Klein, A., Friedrich, U., Vogelsang, H. & Jahreis, G. (2008) Lactobacillus acidophilus 74-2 and Bifidobacterium animalis subsp lactis DGCC 420 modulate unspecific cellular immune response in healthy adults. European Journal of Clinical Nutrition,

Kligler, B., Hanaway, P. & Cohrssen, A. (2007) Probiotics in children. Pediatr Clin N Am,

Klinkenberg, G., Lystad, K. Q., Levine, D. W. & Dyrset, L. (2001) Cell release from alginate immobilized Lactococcus lactis ssp. lactis in chitosan and alginate coated beads. Journal of Dairy Science,

Kneen, M., Farinas, J., Li, Y. & Verkman, A. S. (1998) Green Fluorescent Protein as a Noninvasive Intracellular pH Indicator. Biophysical Journal,

Kodama, M., Sekiguchi, K., Kubo, M., Mitani, K., Osada, M., Sonoda, A., Kashiwazakmi, M. & Saitot, T. (1981) Abstract. 92nd Meeting Jpn Soc Vet Sci.
Kolin, D. L. & Wiseman, P. W. (2007) Advances in image correlation spectroscopy: measuring number densities, aggregation states, and dynamics of fluorescently labeled macromolecules in cells. Cell Biochemistry and Biophyics,

Kollath, W. (1953) Ernahrung und Zahnsystem (Nutrition and the tooth system). Dtsch Zahnarztl Z,

Kosin, B. & Rakshit, S. K. (2006) Microbial and processing criteria for production of probiotics: a review. Food Technology and Biotechnology,

Kremers, G.-J., Gilbert, S. G., Cranfill, P. J., Davidson, M. W. & Piston, D. W. (2011) Fluorescent proteins at a glance. Journal of Cell Science,

Kupikowska, B., Lewinska, D., Dudzinski, K., Jankowska-Sliwinska, J., Grzeczkowicz, M., Wojciechowski, C. & Chwojnowski, A. (2009) Influence of changes in composition of the membrane-forming solution on the structure of alginate-polyethersulfone microcapsules. Biocybernetics and Biomedical Engineering,

Lagoo, A., Tseng, C. K. & Sell, S. (1990) Interleukin 2 produced by activated B lymphocytes acts as an autocrine proliferation-inducing lymphokine. Cytokine,

Lammers, K. M., Bridigi, P., Vitali, B., Gionchetti, P., Rizzello, F., Caramelli, E., Matteuzzi, D. & Campieri, M. (2003) Immunomodulatory effects of probiotic bacteria DNA: IL-1 and IL-10 response in human peripheral blood mononuclear cells. FEMS Immunology and Medical Microbiology,

Landmann, L. (2002) Deconvolution improves colocalization analysis of multiple fluorochromes in 3D confocal data sets more than filtering techniques. Journal of Microscopy,

Lauzurica, P., Sancho, D., Torres, M., Albella, B., Marazuela, M., Merino, T., Bueren, J. A., Martı´Nez-A, C. & Sa´ Nchez-Madrid, F. (2000) Phenotypic and functional characteristics of hematopoietic cell lineages in CD69-deficient mice. Blood,

Lenser, T., Weisshart, K., Ulbricht, T., Klement, K. & Hemmerich, P. (2010) Fluorescence fluctuation microscopy to reveal 3D architecture and function in the cell nucleus. IN SHIVASHANKAR, G. V. (Ed.) Nuclear mechanics and genome regulation. Amsterdam, Netherlands, Elsevier/Academic Press.
Leuko, S., Legat, A., Fendrihan, S. & Stan-Lotter, H. (2004) Evaluation of the Live/Dead BacLight Kit for detection of extremophilic Archaea and visualization of microorganisms in environmental hypersaline samples. Applied and Environmental Microbiology,

Lilly, D. M. & Stillwell, R. H. (1965) Probiotics: growth-promoting factors produced by microorganisms. Science,

Lin, M.-H., Gresshoff, P. M., Indrasumunar, A. & Ferguson, B. J. (2011) pHairyRed: A Novel Binary Vector Containing the DsRed2 Reporter Gene for Visual Selection of Transgenic Hairy Roots. Molecular Plant,
Lin, W.-H., Yu, B., Lin, C.-K., Hwang, W.-Z. & Tsen, H.-Y. (2007) Immune effect of heat-killed multistrain of Lactobacillus acidophilus against Salmonella typhimurium invasion to mice. Journal of Applied Microbiology,

Lindsey, W. B., Lowdell, M. W., Martin, G. E., Abbasi, F., Zenger, V., King, K. M. & Lamb Jr, L. S. (2007) CD69 expression as an index of T-cell function: assay standardization, validation and use in monitoring immune recovery. Cytotherapy,

Liong, M.-T. (2008) Safety of probiotics: translocation and infection. Nutrition Reviews,

Liu, H.-S., Jan, M.-S., Chou, C.-K., Chen, P.-H. & Ke, N.-J. (1999) Is green fluorescent protein toxic to the living cells? Biochemical and Biophysical Research Communications,

Lopez, P., Gueimonde, M., Margolles, A. & Suarez, A. (2010) Distinct Bifidobacterium strains drive different immune responses in vitro. International Journal of Food Microbiology,

Low, B. C. & Gong, Z. (2005) Reporter gene system: green fluorescent protein. In Meyers, R. A. (Ed.) Encyclopedia of Molecular Cell Biology and Molecular Medicine. 2nd ed. Weinheim, Wiley-VCH Verlag GmbH & Co. KGaA.
Lu, J.-Y., Chen, H.-C., Chu, R. Y.-Y., Lin, T.-C. E., Hsu, P.-I., Huang, M.-S., Tseng, C.-J. & Hsiao, M. (2003) Establishment of red fluorescent protein-tagged HeLa tumor metastasis models: Determination of DsRed2 insertion effects and comparison of metastatic patterns after subcutaneous, intraperitoneal, or intravenous injection. Clinical and Medical Metastasis,

Macpherson, A. J. & Harris, N. L. (2004) Interactions between commensal intestinal bacteria and the immune system. Nature Reviews Immunology,

Macpherson, A. J. & Uhr, T. (2004) Induction of protective IgA by intestinal dendritic cells carrying commensal bacteria. Science,

Madsen, K., Cornish, A., Soper, P., Mckaigney, C., Jijon, H., Yachimec, C., Doyle, J., Jewell, L. & De Simone, C. (2001) Probiotic bacteria enhance murine and human intestinal epithelial barrier function. Gastroenterology,

Magde, D., Elson, E. & Webb, W. W. (1972) Thermodynamic fluctuations in a reacting system - measurment by fluorescence correlation spectroscopy. Phys Rev Lett,

Magny, J. P. (2011) New in light of immune modulation by probiotics bacteria and increase IL10 producing regulatory T cells. Archives de Pédiatrie,

Mak, T. W. & Saunders, M. E. (2008) Primer to the immune response, London, UK, Academic Press.
Maldonado Galdeano, C., De Moreno De Leblanc, A., Vinderola, G., Bibas Bonet, M. E. & Perdigon, G. (2007) Proposed model: Mechanisms of immunomodulation induced by probiotic bacteria. Clinical and Vaccine Immunology,

Maldonado Galdeano, C. & Perdigon, G. (2006) The probiotic bacterium Lactobacillus casei induces activation of the gut mucosal immune system through innate immunity. Clinical and Vaccine Immunology,

Malengo, G., Andolfo, A., Sidenius, N., Gratton, E., Zamai, M. & Caiolfa, V.R. (2008) Fluorescence correlation spectroscopy and photon counting histogram on membrane proteins: functionial dynamics of the glycosylphosphatidylinositol-anchored urokinase plasminogen activator receptor. Journal of Biomedical Optics,

Male, D., Brostoff, J., Roth, D. B. & Roitt, I. (2006) Immunology, USA, Mosby.
Mandal, S., Puniya, A. K. & Singh, K. (2006a) Effect of alginate concentration on survival of microencapsulated Lactobacillus casei NCDC-298. International Diary Journal,

Mandal, S., Puniya, A. K. & Singh, K. (2006b) Effect of alginate concentration on survival of microencapsulated Lactobacillus casei NCDC-298. International Diary Journal,

Maqueda, M., Sanchez-Hidalgo, M., Fernandez, M., Montalba N-Lo Pez, M., Valdivia, E. & Martinez-Bueno, M. (2008) Genetic features of circular bacteriocins produced by Gram-positive bacteria. FEMS Microbiology Reviews,

Maruyama, M., Nishio, T., Yoshida, T., Ishida, C., Ishida, K., Watanabe, Y., Nishikawa, M. & Takakura, Y. (2004) Simultaneous detection of DsRed2-tagged and EGFP-tagged human b-interferons in the same single cells. Journal of Cellular Biochemistry,

Matsuzaki, T. & Chin, J. (2000) Modulating immune responses with probiotic bacteria. Immunology & Cell Biology,

Matz, M. V., Fradkov, A. F., Labas, Y. A., Savitsky, A. P., Zaraisky, A. G., Markelov, M. L. & Lukyanov, S. A. (1999) Fluorescent proteins from nonbioluminescent Anthozoa species. Nature Biotechnology,

Mccabe, B. K. (1998) Production of cellulolytic enzymes using immobilised anaerobic fungi. Faculty of Business and Technology. Macarthur, NSW, Australia, University of Western Sydney.
Mcelroy, M., Bouvet, M. & Hoffman, R. M. (2008) Color-coded fluorescent mouse models of cancer cell interactions with blood vessels and lymphatics, San Diego, California, Academic Press.
Medina, M., De Palma, G., Ribes-Konickx, C., Calabuig, M. & Sanz, Y. (2008) Bifidobacterium strains suppress in vitro the pro-inflammatory milieu triggered by the large intestinal microbiota of coeliac patients. Journal of Inflammation,

Medzhitov, R., Preton-Hurlburt, P. & Janeway, C. A. (1997) A human homologue of the Drosophila toll protein signals activation of adaptive immunity. Nature,

Menard, O., Butel, M-J., Gaboriau-Routhiau, V. & & Waligora-Dupriet, A.-J. (2008) Gnotobiotic mouse immune response induced by Bifidobacterium sp. strains isolated from infants. Applied and Environmental Microbiology,

Metchnikoff, E. (1907) Lactic acid as inhibiting intestinal putrefaction. The prolongation of life. Optimistic studies. Heinemann.
Mileti, E., Matteoli, G., Iliev, I. D. & Rescigno, M. (2009) Comparison of the immunomodulatory properties of three probiotic strains of Lactobacilli using complex culture systems: prediction for in vivo efficacy. PLoS One,

4(9):e7056. doi:10.1371/journal.pone.0007056.
Miyagishi, M. & Taira, K. (2002) U6 promoter–driven siRNAs with four uridine 3′ overhangs efficiently suppress targeted gene expression in mammalian cells. Nature Biotechnology,

Miyawaki, A., Sawano, A. & Kogure, T. (2003) Lighting up cells: labelling proteins with fluorophores. Nature Cell Biology,

Moens, P. D. J., Gratton, E. & Salvemini, I. (2011) Fluorescence correlation spectroscopy, raster image correlation spectroscopy, and number and brightnesss on a commercial confocal laser scanning microscopy with analog detectors (Nikon C1). Microscopy Research and Technique,

Mohamadzadeh, M., Olson, S., Kalina, W. V., Ruthel, G., Demmin, G. L., Warfield, K. L., Bavari, S. & Klaenhammer, T. R. (2005) Lactobacilli activate human dendritic cells that skew T cells toward T helper 1 polarization. Proceedings of the National Academy of Sciences,

Moller, G., Sjoberg, O. & Andersson, J. (1973) Immunogenicity, tolerogenicity, and mitogenicity of lipopolysaccharides. The Journal of Infectious Diseases,

Moore, A., Donahue, C. J., Bauer, K. D. & Mather, J. P. (1998) Simultaneous measurement of cell cycle and apoptotic cell death. Methods Cell Biology,

Morelli, L. (2007) In vitro assessment of probiotic bacteria: From survival to functionality. International Dairy Journal,

Mowat, A. M. (2003) Anatomical basis of tolerance and immunity to intestinal antigens. Nature Reviews Immunology,

Müller-Taubenberger, A. (2006) Application of fluorescent protein tags as reporters in live-cell imaging studies. In Eichinger, L. & Rivero, F. (Eds.) Methods in Molecular Biology: Dictyostelium discoideum Protocols. Totowa, NJ, Humana Press Inc.
Nahalkova, J. & Fatehi, J. (2003) Red fluorescent protein (DsRed2) as a novel reporter in Fusarium oxysporum f. sp. lycopersici. FEMS Microbiology Letters,

Naidu, A. S., Bidlack, W. R. & Clemens, R. A. (1999) Probiotic spectra of lactic acid bacteria (LAB). Critical Reviews in Food Science and Nutrition,

Neish, A. S. (2004) Bacterial inhibition of eukaryotic pro-inflammatory pathways. Immunologic Research,

Neurath, M. F., Finotto, S. & Glimcher, L. H. (2002) The role of Th1/Th2 polarization in mucosal immunity. Nature Medicine,

O' Hara, A. M. & Shanahan, F. (2006) The gut flora as a forgotten organ. EMBO Reports,

O'grady, B. & Gibson, G. R. (2005) Microbiota of the human gut. In Tamime, A. (Ed.) Probiotic Dairy Products. Blackwell Publishing Ltd.
Ohtsuka, Y. & Sanderson, I. R. (2000) Transforming growth factor-B: an important cytokine in the mucosal immune response. Current Opinion in Gastroenterology,

Ormo, M., Cubitt, A. B., Kallio, K., Gross, L. A., Tsien, R. Y. & Remington, S. J. (1996) Crystal structure of the aequorea victoria green fluorescent protein. Science,

Oshiba, A. & Gelfand, E. W. (1999) Engagement of the B-cell antigen receptor by antigen negatively regulates IgE production by antigen-specific B cells. Journal of Allergy and Clincal Immunology,

Oswald, I. P. (2006) Role of intestinal epithelial cells in the innate immune defence of the pig intestine. Veterinary Research,

Otte, J.-M. & Podolsky, D. K. (2004) Functional modulation of enterocytes by gram-positive and gram-negative microorganisms. Am J Physiol Gastrointest Liver Physiol,

Paddock, S. W. (2000) Principles and practices of laser scanning confocal microscopy. Molecular Biotechnology,

Paineau, D., Carcano, D., Leyer, G., Darquy, S., Alyanakian, M.-A., Simoneau, G., Bergmann, J.-F., Brassart, D., Bornet, F. & Ouwehand, A. C. (2008) Effects of seven potential probiotic strains on specific immune responses in healthy adults: a double-blind, randomized, controlled trial. FEMS Immunology and Medical Microbiology,

Palmer, C., Bik, E. M., Digiulio, D. B., Relman, D. A. & Brown, P. O. (2007) Development of the human infant intestinal microbiota. PLoS Biology,

Parham, P. (2009) The body's defenses against infection. In Parham, P. (Ed.) The immune system. 3rd ed. New York, USA, Garland Science, Taylor & Francis Group.
Park, J.-H., Um, J.-U., Lee, B.-J., Goh, J.-S., Park, S.-Y., Kim, W.-S. & Kim, P.-H. (2002) Encapsulated Bifidobacterium bifidum potentiates intestinal IgA production. Cellular Immunity,

Parker, R. B. (1974) Probiotics. The other half of the antibodies story. Animal Nutritional Health,

Parra, M. D., Martínez De Morentin, B. E., Cobo, J. M., Mateos, A. & Martínez, J. A. (2004) Daily ingestion of fermented milk containing Lactobacillus casei DN114001 improves innate-defense capacity in healthy middle-aged people. Journal of Physiology and Biochemistry,

Parracho, H., Mccartney, A. L. & Gibson, G. R. (2007) Probiotics and prebiotics in infant nutrition. Proceedings of the Nutrition Society,

Parvez, S., Malik, K. A., Kang, S. A. & Kim, H.-Y. (2006) Probiotics and their fermented food products are beneficial for health. Journal of Applied Microbiology,

Patterson, G. H., Knobel, S. M., Sharif, W. D., Kain, S. R. & Piston, D. W. (1997) Use of the green fluorescent protein and its mutants in quantitative fluorescence microscopy. Biophysics Journal,

Paturi, G., Phillips, M., Jones, M. & Kailasapathy, K. (2007) Immune enhancing effects of Lactobacillus acidophilus LAFTI L10 and Lactobacillus paracasei LAFTI L26 in mice. International Journal of Food Microbiology,

Paturi, G., Phillips, M. & Kailasapathy, K. (2008) Effect of probiotic strains Lactobacillus acidophilus LAFTI L10 and Lactobacillus paracasei LAFTI L26 on systematic immune functions and bacterial translocation in mice. Journal of Food Protection,

Perdigon, G., Vintini, E., Alvarez, S., Medina, M. & Medici, M. (1999) Study of possible mechanisms involved in the mucosal immune system activation by lactic acid bacteria. Journal of Dairy Science,

Pereira, T., Millar, T. J. & Chuck, J.-A. (2005) Viability analysis of alginate encapsulated micro-organisms using fluorescent stains. Journal of Microencapsulation,

Philippe, D., Favre, L., Foata, F., Adolfsson, O., Perruisseau-Carrier, G., Vidal, K., Reuteler, G., Dayer-Schneider, J., Mueller, C. & Blu, S. (2011) Bifidobacterium lactis attenuates onset of inflammation in a murine model of colitis. World Journal of Gastroenterology,

Pilch, D. S., Kirolos, M. A. & Breslauer, K. J. (1995) Berenil binding to higher ordered nucleic acid structures: complexation with a DNA and RNA triple helix? Biochemistry,

Pineiro, M. & Stanton, C. (2007) Probiotic bacteria: legislative framework - requirements to evidence basis. The Journal of Nutrition,

Poch, M. & Bezkorovainy, A. (1988) Growth-enhancing supplements for various species of the genus Bifidobacterium. Journal of Agriculture and Food Chemistry,

Poch, M. & Bezkorovainy, A. (1991) Bovine milk-casein trypsin digest is a growth enhancer for the genus Bifidobacterium Journal of Agriculture and Food Chemistry,

Pochard, P., Gosset, P., Grangette, C., Andre, C., Tonnel, A. B., Pestel, J. & Mercenier, A. (2002) Lactic acid bacteria inhibit TH2 cytokine production by mononuclear cells from allergic patients. Journal of Allergy and Clincal Immunology,

Portugal, J. & Waring, M. J. (1988) Assignment of DNA binding sites for 4',6'-diamidine-2-phenylindole and bisbenzimide (Hoechst 33258). A comparative footprinting study. Biochemicia et Biophysica Acta,

Prado, F. C., Parada, J. L., Pandey, A. & Soccol, C. R. (2008) Trends in non-dairy probiotic beverages. Food Research International,

Prakash, S. & Chang, T. M. S. (1999) Growth kinetics of genetically engineered E. coli DH5 cells in artificial cell APA membrane microcapsules: preliminary report. Artificial cells, blood substitutes, and immobilization technology,

Prasad, J., Gill, H., Smart, J. & Gopal, P.K. (1998) Selection and characterization of Lactobacillus and Bifidobacterium strains for use as probiotics. International Dairy Journal,

Rachmilewitz, D., Katakura, K., Karmeli, F., Hayashi, T., Reinus, C., Rudensky, B., Akira, S., Takeda, K., Lee, J., Takabayashi, K. & Raz, E. (2004) Toll-like receptor 9 signaling mediates the anti-inflammatory effects of probiotics in murine experimental colitis. Gastroenterology,

Ramakrishna, B. S. (2007) The normal bacterial flora of the human intestine and its regulation. Journal of Clinical Gastroenterology,

41(Suppl. 1):S2-S6.
Rasche, C., Wolfram, C., Wahls, M. & Worm, M. (2007) Differential immunomodulating effects of inactivated probiotic bacteria on the allergic immune response. Acta Dermato Venereologica,

Ravishankar, S. & Juneja, V. K. (2000) Sodium chloride. IN NAIDU, A. S. (Ed.) Natural food antimicrobial systems. Florida, USA, CRC Press.
Reggiani, P. C., Heren, C. B., Rimoldi, O. J., Ple'au, J.-M., Dardenne, M. & Goya, R. G. (2006) Gene therapy for long-term restoration of circulating thymulin in thymectomized mice and rats. Gene Therapy,

Remington, S. J. (2006) Fluorescent proteins: maturation, photochemistry and photophysics. Current Opinion in Structural Biology,

Rescigno, M., Urbano, M., Valzasina, B., Francolini, M., Rotta, G., Bonasio, R., Granucci, F., Kraehenbuhl, J.-P. & Ricciardi-Castagnoli, P. (2001) Dendritic cells express tight junction proteins and penetrate gut epithelial monolayers to sample bacteria. Nature Immunology,

Resta-Lenert, S. & Barrett, K. E. (2003) Live probiotics protect intestinal epithelial cells from the effects of infection with enteroinvasive Escherichia coli (EIEC). Gut,

Rieder, F., Brenmoehl, J., Leeb, S., Scholmerich, J. & Rogler, G. (2007) Wound healing and fibrosis in intestinal disease. Gut,

Rizzuto, R., Brini, M., Pizzo, P., Murgia, M. & Pozzan, T. (1995) Chimeric green fluorescent protein as a tool for visualizing subcellular organelles in living cells. Current Biology,

Roberts, A., Matthews, J. B., Socransky, S. S., Freestone, P. P. E., Williams, P. H. & Chapple, I. L. C. (2005) Stress and the periodontal diseases: growth responses of periodontal bacteria to Escherichia coli stress-associated autoinducer and exogenous Fe. Oral Microbiology Immunology,

Romoser, V. A., Hinkle, P. M. & Persechini, A. (1997) Detection in living cells of Ca2+-dependent changes in the fluorescence emission of an indicator composed of two green fluorescent protein variants linked by a calmodulin-binding sequence. The Journal of Biological Chemistry,

Rossnow, M. J., Sasaki, J. M., Digman, M. A. & Gratton, E. (2010) Raster image correlation spectroscopy in live cells. Nature Protocols,

Rudy, J. W., Biedenkapp, J. C., Moineau, J. & Bolding, K. (2006) Anisomycin and the reconsolidation hypothesis. Learning and Memory,

Ruiz, P. A., Hoffman, M., Szcesny, S., Blaut, M. & Haller, D. (2005) Innate mechanisms for Bifidobacterium lactis to activate transient pro-inflammatory host responses in intestinal epithelial cells after the colonization of germ-free rats. Immunology,

Rumbo, M., Anderle, P., Didierlaurent, A., Sierro, F., Debard, N., Sirard, J.-C., Finke, D. & Kraehenbuhl, J.-P. (2004) How the gut links innate and adaptive immunity. Annals New York Academy of Science,

Russell, C., Bhandari, R. R. & Walker, K. (1954) Vitamin requirements of thirty-four lactic acid bacteria associated with brewery products. Journal of General Microbiology,

Russell, J. B., Onodera, R. & Hino, T. (1991) Ruminal protein fermentation: new perspectives on previous contradictions. IN TSUDA, T., SASAKI, Y. & KAWASHIMA, R. (Eds.) Physiological aspects of digestion and metabolism in ruminants: proceedings of the seventh international symposium on ruminant physiology. San Diego, California, Academic Press, Inc.
Sacchetti, A. & Alberti, S. (1999) Protein tags enhance GFP folding in eukaryotic cells. Nature Biotechnology,

SAINSBURY, B. I. (2008) A study of the bioimaging applications of a novel red fluroescent protein isolated from a reef coral. College of Health and Science. Hawkesbury, Australia, University of Western Sydney.
Sakai, K., Mishima, C., Tachiki, T., Kumagi, H. & Tochikura, T. (1987) Mortality of bifidobacteria in boiled yoghurt. Journal of Fermentation Technology,

Salminen, S., Bouley, C., Boutron-Ruault, M.-C., Cummings, J. H., Franck, A., Gibson, G. R., Isolauri, E., Moreau, M.-C., Roberfroid, M. & Rowland, I. (1998a) Functional food science and gastrointestinal physiology and function. British Journal of Nutrition,

80(Suppl. 1):S147-S171.
Salminen, S., Von Wright, A., Morelli, L., Marteau, P., Brassart, D., De Vox, W. M., Fonden, R., Saxelin, M., Collins, K., Mogensen, G., Birkeland, S.-E. & Mattila-Sandholm, T. (1998b) Demonstration of safety of probiotics - a review. International Journal of Food Microbiology,

Sanabria, H., Digman, M. A., Gratton, E. & Waxham, M. N. (2008) Spatial diffusivity and availability of intracellular calmodulin. Biophysical Journal,

Sanders, M.E. (1998). Overview on functional foods: emphasis on probiotic bacteria. International Dairy Journal,

Sanders, M. E. (2006) Probiotics: definition, sources, selection, and uses. Clinical Infectious Diseases,

Schareka, L., Altherra, B. J., Tolkeb, C. & Schmidta, M. F. G. (2007) Influence of the probiotic Bacillus cereus var. toyoi on the intestinal immunity of piglets. Veterinary Immunology and Immunopathology,

Schley, P. D. & Field, C. J. (2002) The immune-enhancing effects of dietary fibres and prebiotics. British Journal of Nutrition,

Schultz, M., Strauch, U. G., Linde, H.-J., Watzl, S., Obermeier, F., Gottl, C., Dunger, N., Grunwald, N., Scholmerich, J. & Rath, H. C. (2004) Preventive effects of Escherichia coli Strain Nissle 1917 on acute and chronic intestinal inflammation in two different murine models of colitis. Clinical and Diagnostic Laboratory Immunology,

Schwille, P. & Haustein, E. (2004) Fluorescence correlation spectroscopy: an introduction to its concepts and applications. Biophysics Journal, http://www.biophysics.org/Portals/1/PDFs/Education/schwille.pdf [Accessed 2.12.10]Seo, E. C., Moon, J. S., Jung, J. Y., Jung, J. Y., Kim, J.-S., Eom, H.-J., Kim, S.-Y., Yoon, H. S. & Han, N. S. (2009) Buffering effects of calcium salts in Kimchi: lowering acidity, elevating lactic acid bacterial population and dextransucrase Activity. Journal of Microbiology and Biotechnology,

Shah, N. P. (2000) Probiotic bacteria: selective enumeration and survival in dairy foods. Journal of Dairy Science,

Shah, N. P. (2007) Functional cultures and health benefits. International Dairy Journal,

Shah, N. P., Lankaputhra, W. E. V., Britz, M. L. & Kyle, S. A. (1995) Survival of Lactobacillus acidophilus and Bifidobacterium bifidum in commercial yoghurt during refrigerated storage. International Dairy Journal,

Shanahan, F. (2004) Probiotics in inflammatory bowel disease - therapeutic rationale and role. Advanced Drug Delivery Reviews,

Sherley, J.L. & Taghizadeh, R.R. (2008) CFP and YFP, but not GFP, provide stable fluorescent marking of rat hepatic adult stem cells. Journal of Biomedicine and Biotechnology, (1), art. no. 453590.
Shimomura, O., Johnson, F.H., & Saiga, Y. (1962) Extraction, purification and properties of aequorinm a bioluminescent protein from the luminous hydromedusan, Aequorea, Journal of Cell Composition and Physiology,

Shu, Q., Qu, F. & Gill, H. S. (2001) Probiotic treatment using Bifidobacterium lactis HN019 reduces weanling diarrhea associated with rotavirus and Escherichia coli infection in a piglet model. Journal of Pediatric Gastroenterology and Nutrition,

Sibirtse, V. S. (2007) Fluorescent DNA probes: study of mechanisms of changes in spectral properties and features of practical application. Biochemistry (Moscow),

Simpson, S. & Wetzler, L. M. (2004) Mucosal Immunity. In Pier, G. B., Lyczak, J. B. & Wetzler, L. M. (Eds.) Immunology, infection, and immunity. Washington, D.C., USA, ASM Press.
Smits, H. H., Engering, A., Van Der Kleij, D., De Jong, E. C., Schipper, K., Van Capel, T. M. M., Zaat, B. A. J., Yazdanbakhsh, M., Wierenga, E. D., Van Kooyk, Y. & Kapsenberg, M. L. (2005) Selective probiotic bacteria induce IL-10-producing regulatory T cells in vitro by modulating dentritic cell function through dentritic cell-specific intercellular adhesion molecule 3-grabbing nonintegrin. Journal of Allergy and Clinical Immunology,

Soska, J. (1966) Growth of Lactobacillus acidophilus in the absence of folic acid. Journal of Bacteriology,

Sperti, G. S. (1971) Probiotics, Westpoint, CT, USA, Avi Publishing Co.
Stadheim, T. A. & Kucera, G. L. (2002) c-Jun N-terminal kinase/stress-activated protein kinase (JNK/SAPK) is required for mitoxantrone- and anisomycin-induced apoptosis in HL-60 cells. Leukemia Research,

Steff, A.-M., Fortin, M., Arguin, C. & Hugo, P. (2001) Detection of a decrease in green fluorescent protein fluorescence for the monitoring of cell death: an assay amenable to high-trhoughput screening technologies. Cytometry,

Steiner, K. & Malke, H. (2000) Life in protein-rich environments: the relA-independent response of Streptococcus pyogenes to amino acid starvation. Molecular Microbiology,

Stocks, S. M. (2004) Mechanism and use of the commercially available viability stain, BacLight. Cytometry,

Strebel, A., Harr, T., Bachmann, F., Wernli, M. & Erb, P. (2001) Green fluorescent protein as a novel tool to measure apoptosis and necrosis. Cytometry,

Strober, W., Fagarasan, S. & Lycke, N. (2005) IgA B cell development. In Mestecky, J., Lamm, M. E., Mcghee, J. R., Bienenstock, J., Mayer, L. & Strober, W. (Eds.) Mucosal Immunology. 3rd ed. Boston, Elsevier Academic Press.
Sumner, J. B., Gralen, N. & Eriksson-Quensel, I.-B. (1938) The molecular weights of urease, canavalin, concanavalin A and concanavalin B. Science,

Suzuka, I., Sekiguchi, K. & Kodama, M. (1985) Some characteristics of a porcine retrovirus from a cell line derived from swin malignant lymphomas. FEBS Letters,

Suzuki, T., Fujikura, K., Higashiyama, T. & Takata, K. (1997) DNA staining for fluorescence and laser confocal microscopy. Journal of Histochemistry and Cytochemistry,

Takagi, A., Matsuzaki, T., Sato, M., Nomoto, K., Morotomi, M. & Yokokura, T. (2001) Enhancement of natural killer cytotoxicity delayed murine carcinogenesis by a probiotic microorganism. Carcinogenesis,

Takeda, K. & Okumura, K. (2007) Effects of a fermented milk drink containing Lactobacillus casei strain Shirota on the human NK-cell activity. The Journal of Nutrition,

Tancrede, C. (1992) Role of human microflora in health and disease. Eur. J. Clin. Microbiol. Infect. Dis.,

Tannock, G. W. (1999) A fresh look at the intestinal microflora. In Tannock, G. W. (Ed.) Probiotics - a critical review. UK, Horizon Scientific Press.
Tao, Y., Drabik, K. A., Waypa, T. S., Musch, M. W., Alverdy, J. C., Schneewind, O., Chang, E. B. & Petrof, E. O. (2006) Soluble factors from Lactobacillus GG activate MAPKs and induce cytoprotective heat shock proteins in intestinal epithelial cells. Am J Physiol Cell Physiol,

Thomson, J. (1979) Lactose metabolism in Streptococcus lactis: phosphorylation of galactose and glucose moeities in vivo. Journal of Bacteriology,

Todah, K. (2009). Nutrition and growth of bacteria. [02.11.11]Tomosaka, H., Omata, S., Hasegawa, E. & Anzai, K. (1997) The effects of substituents introduced into 9-aminoacridine on frameshift mutagenicity and DNA binding affinity. Bioscience, Biotechnology and Biochemistry,

Torii, A., Torri, S., Fujiwara, S., Tanaka, H., Inagaki, N. & Nagai, H. (2007) Lactobacillus acidophilus strain L-92 regulates the production of Th1 cytokine as well as Th2 cytokines. Allergology International,

Tsaia, Y.-T., Chengb, P.-C., Liaod, J.-W. & Pana, T.-M. (2010) Effect of the administration of Lactobacillus paracasei subsp. paracasei NTU 101 on Peyer's patch-mediated mucosal immunity. International Immunopharmacology,

Tsien, R. Y. (1998) The green fluorescent protein. Annual Reviews Biochemistry,

Tuomola, E., Crittenden, R., Playne, M., Isolauri, E. & Salminen, S. (2001) Quality assurance criteria for probiotic bacteria. American Journal of Clinical Nutrition,

Turroni, F., Ribbera, A., Foroni, E., Van Sinderen, D. & Ventura, M. (1998) Human gut microbiota and bifidobacteria: from composition to functionality. Antonie Van Leeuwenhoek,

Uematsu, S. & Akira, S. (2004) Toll-like receptors (TLRs) and their ligands. In Bauer, S. & Hartmann, G. (Eds.) Toll-like receptors (TLRs) and innate immunity. Berlin, Heidelberg, Springer.
Urbanska, A. M., Bhathena, J., Martoni, C. & Prakash, S. (2009) Estimation of the potential antitumor activity of microencapsulated Lactobacillus acidophilus yogurt formulation in the attenuation of tumorigenesis in Apc(Min/+) mice. Digestive Diseases and Sciences,

Urbanska, A. M., Paul, A., Bhathena, J. & Prakash, S. (2010) Suppression of tumorigenesis: modulation of inflammatory cytokines by oral administration of microencapsulated probiotic yogurt formulation. International Journal of Inflammation,

2010:Article ID 894972.
Van Baarlen, P., Troost, F., Van Der Meer, C., Hooiveld, G., Boekschoten, M., Brummer, R. J. M. & Kleerebezem, M. (2011) Human mucosal in vivo transcriptome responses to three lactobacilli indicate how probiotics may modulate human cellular pathways. PNAS,

Vinderola, C. G., Costa, G. A., Regenhardt, S. & Reinheimer, J. A. (2002) Influence of compounds associated withfermented dairy products on the growth of lactic acid starter and probiotic bacteria. International Journal of Dairy Science,

Vinderola, G., Matar, C. & Perdigon, G. (2005) Role of intestinal epithelial cells in immune effects mediated by Gram-positive probiotic bacteria: involvement of toll-like receptors. Clinical and Diagnostic Laboratory Immunology,

Von Der Weid, T., Bulliard, C. & Schiffrin, E. J. (2001) Induction by a lactic acid bacterium of a population of CD4+ T cells with low proliferative capacity that produce transforming growth factor B and interleukin-10. Clinical and Diagnostic Laboratory Immunology,

Walker, W. A. (2008) Mechanisms of action of probiotics. Clinical Infectious Diseases,

Wang, A., Yu, H., Gao, X. & Li, X. (2009) Influence of Lactobacillus fermentum I5007 on the intestinal and systemic immune responses of healthy and E. coli challenged piglets. Antonie Van Leeuwenhoek,

Waring, M. J. (1965) Complex formation between ethidium bromide and nucleic acids. Journal of Molecular Biology,

Winkler, P., Ghadimi, D., Schrezenmeir, J. & Kraehenbuhl, J.-P. (2007) Molecular and cellular basis of microflora-host interactions. The Journal of Nutrition,

WRIGHT, C. T. & KLAENHAMMER, T. R. (1981) Calcium-induced alteration of cellular morphology affecting the resistance of Lactobacillus acidophilus to freezing. Applied and Environmental Microbiology,

Yamamoto, N., Jiang, P., Yang, M., Xu, M., Yamauchi, K., Tsuchiya, H., Tomita, K., Wahl, G. M., Moossa, A. R. & Hoffman, R. M. (2004) Cellular dynamics visualized in live cells in vitro and in vivo by differential dual-color nuclear-cytoplasmic fluorescent-protein expression. Cancer Research,

Yan, F. & Polk, D. B. (2002) Probiotic bacterium prevents cytokine-induced apoptosis in intestinal epithelial cells. The journal of Biological Chemistry,

Yanushevicha, Y. G., Staroverova, D. B., Savitskyb, A. P., Fradkova, A. F., Gurskayaa, N. G., Bulinaa, M. E., Lukyanova, K. A. & Kukyanova, S. A. (2002) A strategy for the generation of non-aggregating mutants of Anthozoa fluorescent proteins. FEBS Letters,

Yasui, H. & Ohwaki, M. (1990) Enhancement of immune response in peyer’s patch cells cultured with Bifidobacterium breve Journal of Dairy Science,

Yokeo, H. & Meyer, T. (1996) Spatial dynamics of GFP-tagged proteins investigated by local fluorescence enhancement. Nature,

Zeuthen, L. H., Fink, L. N. & Frokiaer, H. (2007) Epithelial cells prime the immune response to an array of gut-derived commensals towards a tolerogenic phenotype through distinct actions of thymic stromal lymphopoietin and transforming growth factor-b. Immunology,

Zhang, T. & Fang, H. H. P. (2004) Quantification of Saccharomyces cerevisiae viability using BacLight. Biotechnology Letters,

Zhang, W., Azevedo, M. S. P., Gonzalez, A. M., Saif, L. J., Nguyen, T. V., Wen, K., Yousef, A. E. & Yaun, L. (2008) Influence of probiotic Lactobacilli colonization on neonatal B cell responses in a gnotobiotic pig model of human rotavirus infection and disease. Veterinary Immunology and Immunopathology,

Ziegler, S. F., Levin, S. D., Johnson, L., Copeland, N. G., Gilbert, D. J., Jenkins, N. A., Baker, E., Sutherland, G. R., Feldhaus, A. L. & Ramsdell, F. (1994) The mouse CD69 gene. Structure, expression, and mapping to the NK gene complex. Journal of Immunology,

Zimmer, C. & Wahnert, U. (1986) Nonintercalating DNA-binding ligands: specificity of the interaction and their use as tools in biophysical, biochemical and biological investigations of the genetic material. Prog. Biophys. Mol. Biol.,

Zinchuk, V., Zinchuck, O. & Okada, T. (2007) Quantitative colocalization analysis of multicolor confocal immunofluorescence microscopy images: pushing pixels to explore biological phenomena. Acta Histochem. Cytochem.,

Zipper, H., Brunner, H., Bernhagen, J. & Vitzthum, F. (2004) Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications. Nucleic Acids Research,

Zou, Q., Zhao, J., Liu, X., Tian, F., Zhang, H.-P., Zhang, H. & Chen, W. (2011) Microencapsulg ation of Bifidobacterium bifidum F-35 in reinforced alginate microspheres prepared by emulsification⁄internal gelation. International Journal of Food Science and Technology,


This book is ideally suited for researchers in food and pharmaceutical industries and universities as well as for postgraduate students in microbiology and immunology. This book is a suitable research and teaching text book for advanced microbiology, bacteriology and immunology and is highly recommended for university and research institution libraries around the world.

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