Beta-Galactosidase: Properties, Structure and Functions

Eloy Kras (Editor)

Series: Cell Biology Research Progress
BISAC: SCI017000

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β-galactosidase is an enzyme responsible for catalyzing the hydrolysis of the lactose β-1,4 linkage into α-D-glucose and β-D-galactose.

β-galactosidase is found in plants, animals and microorganisms. In Beta-Galactosidase: Properties, Structure and Functions, the authors discuss the main microorganisms that produce β-galactosidase, the characteristics of the culture media, bioprocessing parameters, the most relevant downstream steps used in the recovery of microbial β-galactosidase, as well as the main immobilization techniques.

Next, this compilation examines β-galactosidases classification, mechanisms, characterization and applications.

Recent advances in galacto-oligosaccharides production and purification are also covered, and different challenges and future perspectives are discussed.

In the closing study, strains with β-Galactosidase activity were isolated from 25 Yak Yoghourt samples collected from the Gannan pasturing area of Gansu Province. An efficient β-galactosidase producing strain SYA2 screened out from 21 strains was identified as Enterobacter sp. by means of morphological feature observation, physiological and biochemical characteristics measurement, and 16S rDNA sequence analysis.
(Imprint: Nova)

Preface

Chapter 1. Microbial βeta-Galactosidase: Production, Bioprocess Parameters and Downstream
(Tatiane A. Gomes; Michele R. Spier, PhD, and Michelle R. F. Vaz, PhD, Chemical Engineering Department, Federal University of Paraná, Curitiba, Paraná, Brazil)

Chapter 2. Survey of B-galactosidases Properties: Applications to Transglycosylation Process
(Cecilia Porciúncula González, Cecilia Giacomini and Gabriela Irazoqui, Laboratorio de Bioquímica, Departamento de Biociencias, Universidad de la República, Montevideo, Uruguay)

Chapter 3. Galacto-Oligosaccharide Synthesis by Transgalactosylation Activity of β-Galactosidase: Recent Trends, Challenges and Future Perspectives
(Milica Simović, Marija Ćorović, Dejan Bezbradica, Ana Milivojević and Katarina Banjanac, Department of Biochemical Engineering and Biotechnology, Faculty of Technology and Metallurgy, University of Belgrade, Belgrade, Serbia, and others)

Chapter 4. Screening and Identification of β-Galactosidase Producing Microorganisms from Yak Yoghourt in China's Gannan Pasture
(Weibing Zhang, Yingying Cao, Kaiyong Wen, Lei Cao, Jiang Ma and Qiaoqiao Luo, College of Food Science and Engineering, Gansu Agricultural University, Lanzhou, China)

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