An Essential Guide to Immunohistochemistry

Kruno Delić (Editor)

Series: Cell Biology Research Progress
BISAC: SCI017000



Volume 10

Issue 1

Volume 2

Volume 3

Special issue: Resilience in breaking the cycle of children’s environmental health disparities
Edited by I Leslie Rubin, Robert J Geller, Abby Mutic, Benjamin A Gitterman, Nathan Mutic, Wayne Garfinkel, Claire D Coles, Kurt Martinuzzi, and Joav Merrick


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Immunohistochemistry has become one of the most reliable techniques to study the expression of different molecules throughout very specific regions, especially in neuroscience. In this compilation, the authors begin by explaining how to perform excellent immunohistochemistry to obtain high quality images, and more importantly, how to automatically analyze these images.

In immunohistochemistry, it is also essential to be able to guarantee analytical reproducibility. By involving experienced observers, data consistency may be improved by increasing interobserver concordance.

Immunohistochemistry has value in melanoma diagnostics, allowing for the distinguishing of malignant melanocytes from benignant ones. This technique also has prognostic relevance in detecting tumor infiltrating lymphocytes and microvascular metastases, discerning the lymphatics from the blood vessels.
(Imprint: Nova)


Chapter 1. Immunohistochemical Imaging and Analysis
(Ramon Guirado, PhD, and Hector Carceller, Cell Biology Department, University of Valencia, Burjassot, Spain)

Chapter 2. Immunohistochemistry Results Assessment – A Scale Based Semiquantitative Approach: The Global Immunohistochemistry Score (GIS)
(Amadeu Borges-Ferro, Lisbon Health School, Lisbon Polytechnic Institute, Lisbon, Portugal)

Chapter 3. Immunohistochemistry in Cutaneous Melanoma
(Luca Roncati, Graziana Gallo and Antonio Manenti, From the Department of Medical and Surgical Sciences, University Hospital of Modena, Modena, Italy)

Chapter 4. Bibliography

Chapter 5. Related Nova Publications


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