Agrobacterium-Mediated Transformation of Sunflower (Helianthus Annuus L.) using Direct Shoot Regeneration Protocol

Ya. B. Neskorodov, А. М. Kamionskaya and K. G. Skryabin
Russian Academy of Sciences, Moscow, Russia

Series: Agricultural Research Updates
BISAC: TEC003000

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Volume 10

Issue 1

Volume 2

Volume 3

Special issue: Resilience in breaking the cycle of children’s environmental health disparities
Edited by I Leslie Rubin, Robert J Geller, Abby Mutic, Benjamin A Gitterman, Nathan Mutic, Wayne Garfinkel, Claire D Coles, Kurt Martinuzzi, and Joav Merrick

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Details

This new book optimizes methods of effective shoots regeneration for a Russian selection of sunflower varieties. A highly effective method of sunflower regeneration in vitro is worked out, where mature embryo (seeds) as explant sources are used. This method enables obtaining four explants with a high regeneration potential from one sunflower seed. The rate of regeneration on the worked out explants ranges from 4583.45 % to 3090.30 % depending on genotype. (Imprint: Novinka)

Abstract

Sterilization method No 1

Sterilization method No 2

Method of Obtaining Explants for Regeneration No 1

Method of Obtaining Explants for Regeneration No 2

Conclusion

References

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