A Comprehensive Guide to Chemiluminescence


Luís Pinto da Silva (Editor)
Chemistry Research Unit (CIQUP), Faculty of Sciences of University of Porto (FCUP), Porto, Portugal

Series: Chemistry Research and Applications
BISAC: SCI007000

Chemiluminescence is a fascinating and mesmerizing phenomenon, which consists on the generation of light as the result of a chemical reaction. This process has been gaining attention in the last decades, being now a standard tool in analytical, bioimaging, biomedical and pharmaceutical applications. This book provides a detailed overview of the basic mechanisms and principles of the most relevant chemiluminescent systems, as well as describing the most recent advances and applications. Written by experts, this is a reference text suitable both for researchers focused on chemiluminescence and for those wanting to start working on this field.

In Chapter One, the progress and evolution of the chemiluminescent studies in imidazopyrazinone-based compounds are reviewed, with emphasis on their potential as diagnosis tools. Chapter Two addresses the spectral changes associated with firefly bioluminescence by focusing on the tautomeric equilibria of the light emitter. Chapter Three reviews, for the first time, the liquid-phase chemiluminescence of fullerene and derivatives. Chapter Four is devoted to studies of chemiluminescence with the participation of lanthanide ions. Chapter Five summarizes the mechanism behind the chemiluminescence of acridinium esters, while discussing their current applications. Chapter Six explains the potential of phenothiazine derivatives as enhancers in analytic chemiluminescent assays. Chapter Seven collects the available information of both well-known and lesser-known examples of squid bioluminescence.

Chapter Eight discusses the enhancement of chemiluminescent emission by using metal-nanoparticles near a luminescent molecule, which is known as metal-enhanced chemiluminescence. In Chapter Nine it is explained how model molecules can make the study of complex bioluminescence reaction mechanisms accessible, as well as allowing for chemiluminescence on macroscopic molecular crystals. Chapter Ten describes experimental and computational approaches used for enhancing the thermostability of firefly luciferase. Chapter Eleven demonstrates the usefulness of theoretical studies for better understanding chemiluminescent reactions. Chapter Twelve describes the fundamental theoretical aspects of state-of-the-art multiconfigurational methods and shows illustrative examples of their application to the study of chemiluminescence. Finally, Chapter Thirteen discusses chemiluminescent experiments that are simple, inexpensive and can be performed by students.
(Imprint: Nova)



Table of Contents


Chapter 1. Chemiluminescent Imidazopyrazinones: A Step to Future Diagnosis Tools
(Ara Núñez-Montenegro, Paulo J.O. Ferreira and Luís Pinto da Silva, Chemistry Research Unit, Faculty of Sciences of University of Porto, Porto, Portugal)

Chapter 2. Emitter Structure in the Firefly Luciferin-Luciferase System: A Historical Perspective
(Natalia N. Ugarova, PhD, and Galina Y. Lomakina, PhD, Department of Chemistry, Lomonosov Moscow State University, Moscow, Russia)

Chapter 3. Chemiluminescence of Fullerenes and Their Derivatives
(Ramil G. Bulgakov and Dim I. Galimov, Institute of Molecule and Crystal Physics, Ufa Federal Research Center, Ufa, Russia, and others)

Chapter 4. Chemiluminescence of Lanthanide Ions
(Ramil G. Bulgakov and Dim I. Galimov, Institute of Molecule and Crystal Physics, Ufa Federal Research Center, Ufa, Russia, and others)

Chapter 5. Computational and Experimental Aspects of Acridinium Esters. Chemiluminescence and Their Applications
(Karol Krzymiński and Beata Zadykowicz, Laboratory of Luminescence Research, Faculty of Chemistry,
University of Gdansk, Gdansk, Poland)

Chapter 6. Phenothiazine Derivatives as Enhancers of Peroxidase-Catalyzed Chemiluminescence and Their Application in Bioanalysis
(Ivan Yu. Sakharov, Department of Chemistry, Lomonosov Moscow State University, Moscow, Russia)

Chapter 7. Bioluminescence in Squids
(Jeremy D. Mirza and Anderson G. Oliveira, Institute of Environmental, Chemical and Pharmaceutical Sciences, Federal University of Sao Paulo, Diadema, Brazil, and others)

Chapter 8. Metal-Enhanced Chemiluminescence
(Joshua Moskowitz and Chris D. Geddes, Institute of Fluorescence and Department of Chemistry and Biochemistry, University of Maryland Baltimore County, Maryland, US)

Chapter 9. Crystalline Fireflies: From Models for Bioluminescence to Solid State Chemiluminescence Reactions
(Stefan Schramm, Friedrich-Schiller University Jena, Germany. New York University Abu Dhabi, UAE)

Chapter 10. Thermostabilization of Firefly Luciferase
(Roghaye Hamidi and Saman Hosseinkhani, Department of Biochemistry, Faculty of Biological Science, Tarbiat Modares University, Tehran, Iran)

Chapter 11. Theoretical Studies of Chemiluminescence
(Cristina García-Iriepa, Romain Berraud-Pache and Isabelle Navizet, Laboratoire Modélisation et Simulation Multi Echelle, Université Paris-Est, MSME, UPEM, Marne-la-Vallée, France, and others)

Chapter 12. Multiconfigurational Quantum Chemiluminescence
(Daniel Roca-Sanjuán, Javier Carmona-García, Miriam Navarrete-Miguel, Antonio Francés-Monerris and Angelo Giussani, Institut de Ciència Molecular, Universitat de València, València, Spain)

Chapter 13. The Art of Chemiluminescence Experiments: Easy to Execute Chemiluminescence Demonstrations as an Educational Tool for Teaching Fundamental Energy Transductions and Attracting High-School Students Towards Chemistry



Chemists; pharmacists; biologists; technicians in food, clinical, toxicological and environmental disciplines; technicians and managers at chemical analytical laboratories; in general, researchers focusing on the study and/or application of chemiluminescence/chemiluminescence-based techniques.


Chemiluminescence, Bioluminescence, Luciferase, Luminescence, Oxyluciferin

Additional information